Background And Objective: Because of their aggressiveness, brain tumors can lead to death within a short time after diagnosis. Optical techniques such as Raman spectroscopy may be a technique of choice for in situ tumor diagnosis, with potential use in determining tumor margins during surgery because of its ability to identify biochemical changes between normal and tumor brain tissues quickly and without tissue destruction.
Methods: In this work, fragments of brain tumor (glioblastoma, medulloblastoma, and meningioma) and normal tissues (cerebellum and meninges) were obtained from excisional intracranial surgery and from autopsies, respectively. Raman spectra (dispersive spectrometer, 830 nm 350 mW, 50 sec accumulation, total 172 spectra) were obtained in vitro on these fragments. It has been developed as a model to discriminate between the spectra of normal tissue and tumors based on the scores of principal component analysis (PCA) and Euclidean distance.
Results: ANOVA indicated that the scores of PC2 and PC3 show differences between normal and tumor groups (p<0.05) which could be employed in a discrimination model. PC2 was able to discriminate glioblastoma from the other tumors and from normal tissues, showing featured peaks of lipids/phospholipids and cholesterol. PC3 discriminated medulloblastoma and meningioma from normal tissues, with the most intense spectral features of proteins. PC3 also discriminated normal tissues (meninges and cerebellum) by the presence of cholesterol peaks. Results indicated a sensitivity and specificity of 97.4% and 100%, respectively, for this in vitro diagnosis of brain tumor.
Conclusions: The PCA/Euclidean distance model was effective in differentiating tumor from normal spectra, regardless of the type of tissue (meninges or cerebellum).
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http://dx.doi.org/10.1089/pho.2012.3460 | DOI Listing |
Ann Nucl Med
January 2025
Turku PET Centre, University of Turku and Turku University Hospital, Turku, Finland.
Dynamic positron emission tomography (PET) can be used to non-invasively estimate the blood flow of different organs via compartmental modeling. Out of different PET tracers, water labeled with the radioactive O isotope of oxygen (half-life of 2.04 min) is freely diffusable, and therefore, very well-suited for blood flow quantification.
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Department of Radiology, Zhengzhou University People's Hospital and Henan Provincial People's Hospital, Zhengzhou, China.
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View Article and Find Full Text PDFAnal Bioanal Chem
January 2025
Molecular Horizons and School of Chemistry and Molecular Bioscience, University of Wollongong, Wollongong, Australia.
The wide range of mass spectrometry imaging (MSI) technologies enables the spatial distributions of many analyte classes to be investigated. However, as each approach is best suited to certain analytes, combinations of different MSI techniques are increasingly being explored to obtain more chemical information from a sample. In many cases, performing a sequential analysis of the same tissue section is ideal to enable a direct correlation of multimodal data.
View Article and Find Full Text PDFChemistry
January 2025
Shanghai Institute of Materia Medica Chinese Academy of Sciences, State Key Laboratory of Drug Research, CHINA.
The fluorescent imaging of pathologically accumulated β-amyloid (Aβ) proteins is of significant importance to the diagnosis of Alzheimer's disease (AD). In the paper, we prepared two new NIR probes, NIR-1 and NIR-2, through hydrophilic modification of introducing water-soluble bioactive groups such as polyethylene glycol (PEG) and morpholine to tune in vivo pharmacokinetics for specific detection of soluble and insoluble Aβ species. The in vitro assessments confirm that both NIR-1 and NIR-2 display strong near-infrared (NIR) fluorescence (FL) enhancement upon association with Aβ42 monomers, oligomers or aggregates (λem > 670 nm) and show high sensitive, rapid and selective response towards Aβ42 species.
View Article and Find Full Text PDFCancer Res Commun
January 2025
University of New Mexico, Albuquerque, NM, United States.
Melanoma brain metastasis (MBM) is linked to dismal prognosis, low overall survival, and is detected in up to 80% of patients at autopsy. Circulating tumor cells (CTCs) are the smallest functional units of cancer and precursors of fatal metastasis. We previously employed an unbiased multilevel approach to discover a unique ribosomal protein large/small subunits (RPL/RPS) CTC gene signature associated with MBM.
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