AI Article Synopsis
- Supported lipid bilayers (SLB) are important for studying lipid membrane processes, but the way they form is still debated due to difficulties in observing individual vesicle events.
- Researchers used interferometric scattering microscopy (iSCAT) to visualize the real-time formation of SLBs from nanoscopic vesicles, capturing phenomena such as vesicle adsorption, rupture, and bilayer spreading.
- Their findings indicate that SLB formation is influenced by the balance between bilayer edge tension and lipid-surface interaction, and iSCAT's capabilities allow for highly sensitive and accurate studies of nanoscale processes.
Article Abstract
Supported lipid bilayers (SLB) are frequently used to study processes associated with or mediated by lipid membranes. The mechanism by which SLBs form is a matter of debate, largely due to the experimental difficulty associated with observing the adsorption and rupture of individual vesicles. Here, we used interferometric scattering microscopy (iSCAT) to directly visualize membrane formation from nanoscopic vesicles in real time. We observed a number of previously proposed phenomena such as vesicle adsorption, rupture, movement, and a wave-like bilayer spreading. By varying the vesicle size and the lipid-surface interaction strength, we rationalized and tuned the relative contributions of these phenomena to bilayer formation. Our results support a model where the interplay between bilayer edge tension and the overall interaction energy with the surface determine the mechanism of SLB formation. The unique combination of sensitivity, speed, and label-free imaging capability of iSCAT provides exciting prospects not only for investigations of SLB formation, but also for studies of assembly and disassembly processes on the nanoscale with previously unattainable accuracy and sensitivity.
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| http://dx.doi.org/10.1021/nn403367c | DOI Listing |
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