The increased concentration of glutamate in synaptic vesicles, mediated by the vesicular glutamate transporter (VGLUT), is an initial vital step in glutamate synaptic transmission. Evidence indicates that aberrant overexpression of VGLUT is involved in certain pathophysiologies of the central nervous system. VGLUT is subject to inhibition by various types of agents. The most potent VGLUT-specific inhibitor currently known is Trypan Blue, which is highly charged, hence membrane-impermeable. We have sought a potent, VGLUT-specific agent amenable to easy modification to a membrane-permeable analog. We provide evidence that Brilliant Yellow exhibits potent, VGLUT-specific inhibition, with a Ki value of 12 nM. Based upon structure-activity relationship studies and molecular modeling, we have defined the potent inhibitory pharmacophore of Brilliant Yellow. This study provides new insight into development of a membrane-permeable agent to lead to specific blockade, with high potency, of accumulation of glutamate into synaptic vesicles in neurons.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4025951 | PMC |
| http://dx.doi.org/10.1007/s11064-013-1196-8 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Michael Maier's Medicament Coelidonia - A Possible Explanation of its Composition and Production.
Ambix
November 2024
Independent Researcher, Kassel, Germany.
The composition and way of production of Michael Maier's medicament Coelidonia, mentioned in his book were inferred from indications in Maier's book . The substance was synthesized by the author in a modern laboratory. It is a lead oxychloride of brilliant yellow colour with a composition of approximately PbOCl.
View Article and Find Full Text PDFDegradation of Procion brilliant yellow H-E6G using ultrasonic and hydrodynamic cavitation combined with oxidants with demonstration at pilot scale.
Water Environ Res
March 2024
Chemical Engineering Department, Institute of Chemical Technology, Mumbai, India.
The current study focuses on the degradation of Procion brilliant yellow H-E6G, an azo dye, using ultrasonic and hydrodynamic cavitation (HC), evaluating the impact of various parameters on the extent of degradation. The use of only ultrasound showed less oxidation capacity as indicated by only 19.1% degradation at an optimized power of 140 W, pH of 2.
View Article and Find Full Text PDFRP-HPLC Separation and H NMR Identification of a Yellow Fluorescent Compound-Riboflavin (Vitamin B)-Produced by the Yeast .
Biomolecules
September 2023
Departamento de Ingeniería Bioquímica, Instituto Politécnico Nacional, Escuela Nacional de Ciencias Biológicas, Avenida Wilfrido Massieu s/n, Unidad Profesional Adolfo López Mateos, Ciudad de Mexico 07738, Mexico.
The yeast excretes a brilliant yellow fluorescent compound into its growth culture. In this study, we isolated and identified this compound using reverse-phase high-performance liquid chromatography-diode array detector (RP-HPLC-DAD) as well as H NMR and UV-Vis spectroscopy. Two of the three RP-HPLC-DAD methods used successfully separated the fluorescent compound and involved (1) a double separation step with isocratic flow elution, first on a C18 column and later on a cyano column, and (2) a separation with a linear gradient elution on a phenyl column.
View Article and Find Full Text PDFReversible Electrochromic/Electrofluorochromic Dual Switching in Zn(II)-Based Metallo-Supramolecular Polymer Films.
ACS Appl Mater Interfaces
September 2023
Electronic Functional Macromolecules Group, National Institute for Materials Science (NIMS), 1-1 Namiki, Tsukuba 305-0044, Japan.
The introduction of novel materials with multifunctional chromogenic properties, such as electrochromic/electrofluorochromic (EC/EFC) properties, has recently attracted prospective interest in the development of various optoelectronic devices and smart windows. In this study, a novel Zn(II)-based metallo-supramolecular polymer () has been developed as an ON/OFF switchable EFC application with prominent EC behavior. In this regard, the polymeric chain of was first synthesized by 1:1 complexation in a zigzag manner with Zn(II) ions at the metal center and 4,4'-[bis(2,2':6',2″-terpyridinyl)benzene]triphenylamine () as the redox-active ditopic ligand.
View Article and Find Full Text PDFLychaete pellucida as a novel biosorbent for the biodegradation of hazardous azo dyes.
Environ Monit Assess
July 2023
Botany and Microbiology Department, Faculty of Science, Damanhour University, Damanhour, Egypt.
The majority of textile wastes are made up of toxic dyes. Additionally, because these compounds are soluble, wastewater may include significant concentrations. In this work, the green alga Lychaete pellucida is used for the bioremoval of four common azo dyes, Reactive Blue 4 (RB4), Reactive Red 120 (RR120), Reactive Brilliant Yellow 3G (RBY3G), and Reactive Green12 (RG12), with the application of two models of sorption isotherms, Langmuir and Freundlich.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!