The growth of Distichlis spicata suspension cultures in LS medium without NaCl was inhibited 54% by 2 mM proline. In medium containing 260 mM NaCl, 10 mM proline inhibited growth by only 22%. The uptake and metabolism of 10 mM L-[1-(13)C] proline was followed by (13)C NMR and ninhydrin analyses of suspensions cultured in the presence of 0 or 260 mM NaCl. Uptake of 85 to 92% of the exogenous proline occurred within 72 h in all media. In 10 mM proline and no NaCl, cellular proline reached a maximm of 51.5 μmoles/g FW compared to 1.9 μmoles/g FW in suspensions not grown on proline. In medium containing 260 mM NaCl and proline, cellular proline reached 59-65 μmoles/g FW compared to 30-40 μmoles/g FW in controls grown without proline. The (13)C-label in the proline-C1 was either retained in proline or disappeared, presumably released as carbon dioxide, by catabolism through the TCA cycle. Since no metabolite of (13)C-proline was detected by NMR, proline was considered to be the molecule which inhibited the suspension culture growth.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/BF00269924 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Physiological and transcriptomic analysis of the effect of overexpression of the NTPIP2;4 gene on drought tolerance in tobacco.
BMC Plant Biol
January 2025
College of Tobacco Science, Guizhou University, Guiyang, 550025, China.
Aquaporins are widely present in the plant kingdom and play important roles in plant response to abiotic adversity stresses such as water and temperature extremes. In this study, we investigated the regulatory role of NTPIP2;4 on drought tolerance in tobacco at physiological and transcriptional levels. In this experiment, we constructed an NtPIP2;4 overexpression vector and genetically transformed tobacco variety 'K326' to investigate the mechanism of NtPIP2;4 gene in regulating drought tolerance in tobacco at physiological and transcriptomic levels.
View Article and Find Full Text PDFA reliable LC-MS/MS method for the quantification of natural amino acids in human plasma and its application in clinic.
J Pharm Biomed Anal
January 2025
Institute of Drug Metabolism and Pharmaceutical Analysis, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China; Jinhua Institute of Zhejiang University, Jinhua 321036, China; State Key Laboratory of Advanced Drug Delivery and Release Systems, Zhejiang University, Hangzhou 310058, China. Electronic address:
A simple and fast LC-MS/MS method was developed and validated for simultaneous quantification of 20 L-amino acids (AAs) in human plasma. Chromatographic separation was achieved on an Agilent AdvanceBio Hilic column within 15 min via gradient elution with an aqueous solution containing 5 mM ammonium formate, 5 mM ammonium acetate and 0.1 % formic acid and an organic mobile phase containing 0.
View Article and Find Full Text PDFA Protocol for GC-MS Profiling of Chiral Secondary Amino Acids.
Methods Mol Biol
January 2025
Laboratory of Analytical Biochemistry & Metabolomics, Biology Centre, Czech Academy of Sciences, České Budějovice, Czech Republic.
A simple analytical workflow is described for gas chromatographic-mass spectrometric (GC-MS)-based chiral profiling of secondary amino acids (AAs) in biological matrices. The sample preparation is carried out directly in aqueous biological sample extracts and involves in situ heptafluorobutyl chloroformate (HFBCF) derivatization-liquid-liquid microextraction of nonpolar products into hexane phase followed by subsequent formation of the corresponding methylamides from the HFB esters by direct treatment with methylamine reagent solution. The (O, N) HFB-butoxycarbonyl-methylamide AA products (HFBOC-MA) are separated on a Chirasil-L-Val capillary column and quantitatively measured by GC-MS operated in selected ion monitoring (SIM) mode.
View Article and Find Full Text PDFChemical and Enzymatic Mechanosynthesis of Organocatalytic Peptide Materials Based on Proline and Phenylalanine.
ChemSusChem
January 2025
Universidad de Antioquia Facultad de Ciencias Exactas y Naturales, Instituto de Química, Calle 70 No 52-21, Medellín, NA, Medellín, COLOMBIA.
In recent years, mechanosynthesis of peptides through either chemical or enzymatic routes has been accomplished. In part, this advancement has been driven due to the organocatalytic properties of peptide-based biomaterials. In this work, we report the merging of chemical and enzymatic protocols under mechanochemical conditions to synthesize peptide materials based on L-proline and L-phenylalanine.
View Article and Find Full Text PDFOverexpression of the Vitis amurensis Ca-binding protein gene VamCP1 in Arabidopsis thaliana and grapevine improves cold tolerance.
Physiol Plant
January 2025
College of Enology and Horticulture, Ningxia University/College of Modern Grape and Wine Industry/Ningxia Grape and Wine Research Institute/Engineering Research Center of Grape and Wine, Ministry of Education, Yinchuan, P.R. China.
Calcium ions (Ca) are important second messengers and are known to participate in cold signal transduction. In the current study, we characterized a Ca-binding protein gene, VamCP1, from the extremely cold-tolerant grape species Vitis amurensis. VamCP1 expression varied among organs but was highest in leaves following cold treatment, peaking 24 h after treatment onset.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!