Copepods of the genusEurytemora, isolated from the Patuxent River, a tributary of Chesapeake Bay, were fed suspensions of the ciliateUronema isolated from the Rhode River, a subestuary of Chesapeake Bay. Grazing by copepods was determined by the decrease in numbers of ciliates, which were monitored by both direct counting and particle size analysis. Results from both methods of analysis showed significant reduction in the numbers ofUronema in the suspension whenEurytemora was present. Survival of copepods with ciliates added as food source was significantly longer than without ciliates. Analysis of field samples collected in the fall showed that ciliates comprised approximately 20% of the total plankton biomass at selected sampling sites. The results of the laboratory and field studies indicate that copepods can feed on ciliates and suggest that, in nature, ciliates may comprise an important source of food for copepods.
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Sci Total Environ
January 2025
Institute of Marine Environment and Ecology, National Taiwan Ocean University, Keelung 202-24, Taiwan; Doctoral Degree Program in Ocean Resource and Environmental Changes, National Taiwan Ocean University, Keelung 202-24, Taiwan; Center of Excellence for the Oceans, National Taiwan Ocean University, Keelung 202-24, Taiwan. Electronic address:
Extreme weather events, such as heavy rainfall and typhoons, are becoming more frequent due to climate change and can significantly impact coastal microbial communities. This study examines the short-term alterations in microbial food webs-viruses, bacteria, picophytoplankton, nanoflagellates, ciliates, and diatom-following Typhoon Krathon in Taiwan's coastal waters in October 2024. Daily in situ sampling revealed a significant post-typhoon increased in viral, nanoflagellate, and Synechococcus spp.
View Article and Find Full Text PDFMar Biotechnol (NY)
January 2025
Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture and Rural Affairs, Jimei University, Xiamen, China.
Large yellow croaker (Larimichthys crocea) is facing various threats from bacterial, viral, and parasitic diseases, especially scuticociliate. Scuticociliate is a facultative parasite causing high mortality in various marine fishes. In this study, an artificial scuticociliate infection model was successfully established for large yellow croaker.
View Article and Find Full Text PDFACS Pharmacol Transl Sci
January 2025
Division of Applied Regulatory Science, Office of Clinical Pharmacology, Office of Translational Sciences, Center for Drug Evaluation and Research, United States Food and Drug Administration (FDA), Silver Spring, Maryland 20993, United States.
Current in vitro cell-based methods, relying on single cell types, have structural and functional limitations in determining lung drug permeability, which is a contributing factor affecting both local and systemic drug levels. To address this issue, we investigated a 3D human lung airway model generated using a cell culture insert, wherein primary human lung epithelial and endothelial cells were cocultured at an air-liquid interface (ALI). To ensure that the cell culture mimics the physiological and functional characteristics of airway tissue, the model was characterized by evaluating several parameters such as cellular confluency, ciliation, tight junctions, mucus-layer formation, transepithelial electrical resistance, and barrier function through assaying fluorescein isothiocyanate-dextran permeability.
View Article and Find Full Text PDFProtoplasma
January 2025
Core Facility Center "Cultivation of Microorganisms", Saint-Petersburg State University, Saint-Petersburg, Russian Federation.
Ciliates often form symbiotic associations with other microorganisms, both prokaryotic and eukaryotic. We are now starting to rediscover the symbiotic systems recorded before molecular analysis became available. Here, we provide a morphological and molecular characterization of a symbiotic association between the ciliate Paramecium tritobursaria and the yeast Rhodotorula mucilaginosa (syn.
View Article and Find Full Text PDFYing Yong Sheng Tai Xue Bao
October 2024
Liaoning Ocean and Fisheries Science Research Institute/Key Laboratory of Protection and Utilization of Aquatic Germplasm Resource, Ministry of Agriculture and Rural Affairs/Key Laboratory of Molecular Biology for Marine Fishery, Dalian 116023, Liaoning, China.
We investigated food composition and feeding selectivity of jellyfish () from the coastal aquaculture ponds in Liaodong Bay by DNA metabarcoding technology. The DNA from environmental water samples and stomach contents of were extracted and sequenced by high-throughput sequencing with 18S rDNA V4 region and mitochondrial cytochrome c oxidase subunit I (COI) as metabarcoding markers. Based on 18S rDNA metabarcoding, we detected 27 phyla in the stomach contents of , in which Mollusc was the dominant phylum followed by Arthropod, and 34 phyla in the environmental water samples, in which Pyrrophyta was the dominant phylum followed by Ciliophora and Ascomycota.
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