Sensory organelle cilia have critical roles in mammalian embryonic development and tissue homeostasis. Intraflagellar transport (IFT) machinery is required for the assembly and maintenance of cilia. Yet, how this large complex passes through the size-dependent barrier at the ciliary base remains enigmatic. Here we report that FBF1, a highly conserved transition fibre protein, is required for the ciliary import of assembled IFT particles at the ciliary base. We cloned dyf-19, the Caenorhabditis elegans homologue of human FBF1, in a whole-genome screen for ciliogenesis mutants. DYF-19 localizes specifically to transition fibres and interacts directly with the IFT-B component DYF-11/IFT54. Although not a structural component of transition fibres, DYF-19 is required for the transit of assembled IFT particles through the ciliary base. Furthermore, we found that human FBF1 shares conserved localization and function with its worm counterpart. We conclude that FBF1 is a key functional transition fibre component that actively facilitates the ciliary entry of assembled IFT machinery.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3856926 | PMC |
| http://dx.doi.org/10.1038/ncomms3750 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Probing the Self-Assembly dynamics of cellulose nanocrystals by X-ray photon correlation spectroscopy.
J Colloid Interface Sci
December 2024
Wallenberg Wood Science Center, KTH Royal Institute of Technology, SE-100 44 Stockholm, Sweden; Department of Fibre and Polymer Technology, KTH Royal Institute of Technology, SE-100 44 Stockholm, Sweden. Electronic address:
Hypothesis: Charge-stabilized colloidal cellulose nanocrystals (CNCs) can self-assemble into higher-ordered chiral nematic structures by varying the volume fraction. The assembly process exhibits distinct dynamics during the isotropic to liquid crystal phase transition, which can be elucidated using X-ray photon correlation spectroscopy (XPCS).
Experiments: Anionic CNCs were dispersed in propylene glycol (PG) and water spanning a range of volume fractions, encompassing several phase transitions.
Ln-MOF in production of durable antimicrobial and UV-Protective fluorescent cotton fabric for potential application in military textiles.
Sci Rep
January 2025
Chemistry Department, Faculty of Science, Helwan University, Ain-Helwan, Cairo, 11795, Egypt.
Industrialization of military textiles faces many challenges and some requirements such as durability, protection and suitability for hostile environment must be provided. Herein, fluorescent protective cotton with ultraviolet radiation (UVR)-protection and antimicrobial property was currently prepared via the immobilization of lanthanide-metal organic framework (Ln-MOF). Cotton fabrics were primarily activated via cationization process with 3-Chloro-2-hydroxypropyltrimethyl ammonium chloride to obtain the cationized cotton (Q-cotton).
View Article and Find Full Text PDFThermodynamics of nucleosome breathing and positioning.
J Chem Phys
January 2025
Department of Physics and Astronomy and Center for Quantitative Biology, Rutgers University, Piscataway, New Jersey 08854, USA.
Nucleosomes are fundamental units of chromatin in which a length of genomic DNA is wrapped around a histone octamer spool in a left-handed superhelix. Large-scale nucleosome maps show a wide distribution of DNA wrapping lengths, which in some cases are tens of base pairs (bp) shorter than the 147 bp canonical wrapping length observed in nucleosome crystal structures. Here, we develop a thermodynamic model that assumes a constant free energy cost of unwrapping a nucleosomal bp.
View Article and Find Full Text PDFRecent Advancements in CoO-Based Composites for Enhanced Electrocatalytic Water Splitting.
Micromachines (Basel)
November 2024
Department of Fiber System Engineering, Yeungnam University, 280 Dehak-Ro, Gyeongsan 38541, Republic of Korea.
The pursuit of efficient and economical catalysts for water splitting, a critical step in hydrogen production, has gained momentum with the increasing demand for sustainable energy. Among the various electrocatalysts developed to date, cobalt oxide (CoO) has emerged as a promising candidate owing to its availability, stability, and catalytic activity. However, intrinsic limitations, including low catalytic activity and poor electrical conductivity, often hinder its effectiveness in electrocatalytic water splitting.
View Article and Find Full Text PDFStudy on the Curing Behaviors of Benzoxazine Nitrile-Based Resin Featuring Fluorene Structures and the Excellent Properties of Their Glass Fiber-Reinforced Laminates.
Materials (Basel)
December 2024
School of Materials and Energy, University of Electronic Science and Technology of China, Chengdu 610054, China.
Benzoxazine and o-phthalonitrile resin are two of the most eminent polymer matrices within high-performance fiber-reinforced resin-based composite materials. Studying the influence modalities of their structures and forming processes on performance can furnish a theoretical basis for the design and manufacturing of superior performance composite materials. In this study, we initially incorporated a fluorene structure into the molecular main chain through molecular design to prepare a fluorene-containing benzoxazine nitrile-based resin.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!