The chicken gene for transcription factor CTCF was expressed in COS-1 mammalian cells. The CTCF protein containing polyhistidine tag was partially purified using metallo-affinity and ion-exchange chromatography. The expressed protein localized in the cell nucleus and was shown to be functionally active in the electrophoretic mobility shift assay and specifically interacted with anti-CTCF antibodies.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1134/S0006297913080038 | DOI Listing |
Publication Analysis
Top Keywords
ctcf protein
8
expression chicken
4
ctcf
4
chicken ctcf
4
ctcf gene
4
gene cos-1
4
cos-1 cells
4
cells partial
4
partial purification
4
purification ctcf
4
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!