AI Article Synopsis
- Researchers developed a method to distinguish Astragali Radix from Hedysari Radix using PCR to amplify specific DNA alleles from 30 and 28 samples, respectively.
- The study involved extracting DNA, amplifying specific sequences (trnL-trnF), and optimizing PCR conditions; results showed distinct band sizes for each plant – 136 bp for Astragali Radix and 323 bp for Hedysari Radix.
- This technique effectively identifies and authenticates these plant materials, allowing for detection of 10% Hedysari Radix DNA mixed in with Astragali Radix.
Article Abstract
To explore the new method of discriminating Astragali Radix and Hedysari Radix by using PCR amplification of specific alleles, 30 samples of the different Astragali Radix materials and 28 samples of Hedysari Radix were collected. The total DNA of all samples were extracted, trnL-trnF sequence from Astragali Radix and Hedysari Radix was amplified by PCR and sequenced unidirectionally. These sequences were aligned by using Clustul W. Primer was designed and the PCR reaction systems including annealing temperature, dNTP, etc were optimized. All samples were amplified by PCR with specific primer, DNA from Astragali Radix would be amplified 136 bp, whereas PCR products from all of Hedysari Radix were 323 bp. This method can detect 10% of intentional Hedysari Radix DNA into Astragali Radix. PCR amplification of alleles can be used to identify Astragali Radix and Hedysari Radix successfully and is an efficient molecular marker for authentication of Astragali Radix and Hedysari Radix.
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