AI Article Synopsis
- The study aims to differentiate Gelsemium elegans from Lonicera japonica and related species through specific PCR amplification.
- Thirteen G. elegans samples and 58 samples from various Lonicera species were collected, and DNA was extracted and amplified using PCR techniques.
- The results showed that PCR effectively amplified a specific 97 bp DNA fragment for G. elegans, while no bands were produced for Lonicera samples, confirming the method's efficacy as a molecular marker for identification.
Article Abstract
Objective: To explore the new method of discriminating Gelsemium elegans from Lonicera japonica and its close species by using specific PCR amplification.
Method: Thirteen samples of the different G. elegans materials and 58 samples of L. japonica, L. macranthoides and L. dasystyla were collected. The total DNA of the samples were extracted, and the DNA of G. elegans, L. japonica and L. macranthoides water extracts were extracted. PsbA-rnnH sequence from G. elegans was amplified by PCR and sequenced unidirectionally, ClustulW was used to align psbA-trnH sequences of the G. elegans and L. japonica and its close species from GenBank database.
Result: All samples were amplified by PCR with specific primer, DNA from G. elegans would be amplified 97 bp whereas PCR products from all of Lonicera samples had not bands.
Conclusion: Specific PCR amplification can be used to identify G. elegans from L. japonica and its close species successfully and is an efficient molecular marker for authentication of G. elegans and L. japonica and its close species.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!