AI Article Synopsis
- Transposon mutant libraries are essential tools for studying hard-to-manipulate bacteria like Staphylococcus epidermidis, which currently lacks a sequence-defined mutant library.
- Previous research on S. aureus has laid the groundwork, but S. epidermidis has unique traits, particularly in how it adheres and forms biofilms.
- The study discusses modifications to existing methods for creating mutants in S. epidermidis using a specific transposon called bursa aurealis.
Article Abstract
Transposon mutant libraries are valuable resources to investigators studying bacterial species, including Staphylococcus epidermidis, which are difficult to genetically manipulate. Although sequence-defined transposon mutant libraries have been constructed in Staphylococcus aureus, no such library exists for S. epidermidis. Nevertheless, the study of Tn917-mediated mutations has been paramount in discovering unique aspects of S. epidermidis biology including initial adherence and accumulation during biofilm formation. Herein, we describe modifications to the methodology first described by Bae et al. to utilize the mariner-based transposon bursa aurealis to generate mutants in S. epidermidis strain 1457.
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| http://dx.doi.org/10.1007/978-1-62703-736-5_12 | DOI Listing |
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