Flux variability analysis: application to developing oilseed rape embryos using toolboxes for constraint-based modeling.

Methods Mol Biol

Biosciences Department, Brookhaven National Laboratory, Upton, NY, USA.

Published: July 2014

Flux variability analysis enables comprehensive exploration of alternate optimal routes in a metabolic network. This method is especially useful with models such as bna572 for the developing oilseed rape embryo which is highly compartmentalized. Here, we describe a protocol for carrying out flux variability analysis on reactions and network projections of bna572 using well-established software (CellNetAnalyzer and COBRA) for constraint-based analysis of stoichiometric network reconstructions.

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http://dx.doi.org/10.1007/978-1-62703-688-7_18DOI Listing

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