Eighteen calves were orally inoculated with either 200,000 or 225,000 sporocysts of Sarcocystis cruzi. Eight goats were orally inoculated with 20,000 sporocysts of S capracanis. Calves and goats were euthanatized at various times after inoculation, and portions of their right and left biceps femoris, right and left longissimus dorsi, myocardium, and tongue were frozen at -150 C in precooled isopentane and stored at -70 C. Frozen sections of these muscles were stained with hematoxylin and eosin, modified Gomori's trichrome, nonspecific esterase, diphosphopyridine nucleotide tetrazolium reductase, and adenosinetriphosphatase at pH 10.4 and 4.6. Muscle from the same locations was fixed in 10% neutral buffered formalin, processed for paraffin embedding, sectioned, and stained with hematoxylin and eosin. Microscopic examination of both calf and goat tissue indicated that both type I and type II muscle fibers were equally infected and that infected myofibers showed no apparent damage other than displacement by sarcocysts. Occasionally, muscle fibers within the muscle spindles contained sarcocysts.
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Parasit Vectors
December 2024
United States Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Centre, Animal Parasitic Diseases Laboratory, Beltsville, MD, 20705-2350, USA.
Background: Parasites in the apicomplexan genus Sarcocystis infect cattle worldwide. Assessing the economic importance of each such parasite species requires proper diagnosis. Sarcocystis cruzi, a thin-walled species, infects virtually all cattle.
View Article and Find Full Text PDFBMC Vet Res
December 2024
Zoology and Entomology Department, Faculty of Science, Al-Azhar University (Girl's Branch), Cairo, Egypt.
Background: Sarcocystosis is a parasitic disease found worldwide, resulting from various Sarcocystis species. The current research was carried out in three significant economic areas in Egypt: Greater Cairo, the Nile Delta, and Upper Egypt. It aimed to investigate the occurrence of Sarcocystis spp.
View Article and Find Full Text PDFJ Parasitol
December 2024
U.S. Department of Agriculture, Agricultural Research Service, Animal Parasitic Diseases Laboratory, Beltsville Agricultural Research Center, Building 1001, Beltsville, Maryland 20705.
As predators of a wide variety of livestock and wildlife species, wolves (Canis lupus) could serve as definitive hosts for species of Sarcocystis parasites infecting many intermediate hosts. Sarcocystis sporocysts have been observed in the feces of wolves fed infected beef before, but genetic tools have not yet definitively identified which species of parasites they harbor, impairing understanding of their contribution to infections in wild and domesticated intermediate hosts. Therefore, we genetically characterized sporocysts derived from a small sample of naturally infected wolves in Minnesota.
View Article and Find Full Text PDFActa Trop
September 2024
Department of Veterinary Medicine, University of Bari, Valenzano, Bari, Italy; Department of Veterinary Clinical Sciences, City University of Hong Kong, SAR, China. Electronic address:
Colpodella species are close relatives of Apicomplexan protozoa. Although most species of this genus are free-living organisms that feed on other protists and algae, reports indicate their occurence in ticks and human patients, including an individual with a history of tick bite manifesting neurological symptoms. During an investigation of tick-borne pathogens (TBPs) in blood samples of cattle, goats, and in ticks collected on them, Colpodella sp.
View Article and Find Full Text PDFFront Vet Sci
June 2024
Nature Research Centre, Vilnius, Lithuania.
Background: includes a global group of apicomplexan parasites with two-host life cycle frequently circulating in wildlife and domestic hosts, including humans. Two of the most important wild terrestrial carnivores acting as definitive hosts are the red fox and raccoon dog, due to their wide distribution in Europe and usage of wild and farmed animals as prey. This study was conducted to determine the prevalence of in hunted red foxes and raccoon dogs from nine regions of the Czech Republic and to identify isolated sporocysts by molecular techniques.
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