Using MALDI-TOF mass spectrometry (MS) peptide fingerprinting procedure followed by the analysis of MS data with the GlycoMod tool from the ExPASy proteomic site, N-glycosylation of two GH51 and GH54 family α-l-arabinofuranosidases (Abf51A and Abf54A) from Penicillium canescens was studied. Variable N-linked glycans were identified at five out of eight potential N-glycosylation sites in the Abf51A and one out of three potential N-glycosylation sites in the Abf54A. The discriminated glycans represented high-mannose oligosaccharides (Man)x(GlcNAc)2 with a number of Man residues up to 7 or the products of sequential enzymatic trimming of a high-mannose glycan with α-mannosidases and β-N-acetylhexosaminidases. The Abf54A peptide, containing the Asn254 glycosylation site, and one peptide from the Abf51A, containing the Asn163 glycosylation site, were found to exist not only in glycosylated, but also in a native non-modified form.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.carres.2013.08.026 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Flexibility of active center affects thermostability and activity of Penicillium canescens xylanase E.
Biochimie
January 2024
Federal Research Centre «Fundamentals of Biotechnology», Russian Academy of Sciences, Moscow, 119071, Russia; Department of Chemistry, M.V. Lomonosov Moscow State University, Moscow, 119991, Russia. Electronic address:
Xylanases are used in several industrial applications, such as feed additives, the bleaching of pulp and paper, and the production of bread, food, and drinks. Xylanases are required to remain active after heat treatment at 80-90 °C for 30 s to several minutes due to the conditions of feed pelleting. Also, xylanases need to be active at 60-70 °C for several hours while bleaching of pulp and paper or manufacturing of bread, food, and drinks is performed.
View Article and Find Full Text PDFChemical characterization and toxicity evaluation of fungal pigments for potential application in food, phamarceutical and agricultural industries.
Saudi J Biol Sci
May 2023
Department of Biotechnology and Food Technology, Arcadia Campus, Tshwane University of Technology, Pretoria, Private Bag X680, Pretoria 0001, South Africa.
Concerns associated with the use of synthetic colourants backs the demand for natural colourants. Thus, the current study aimed at characterizing crude fungal pigments produced by Penicillium multicolour, P. canescens, Talaromyces verruculosus, Fusarium solani and P.
View Article and Find Full Text PDFTwo pairs of new isobenzofuranone enantiomers from a soil-derived fungus DWS225.
Nat Prod Res
August 2024
Xiangya School of Pharmaceutical Sciences, Central South University, Changsha, Hunan, PR China.
Article Synopsis
- - Two new pairs of isobenzofuranone derivative enantiomers, named (±)-penicifurans E and F, along with four known compounds, were isolated from DWS225 during solid fermentation.
- - The structures of these enantiomers were determined using detailed NMR spectroscopy, and their configurations were confirmed through experimental and calculated ECD data.
- - The neuroprotective effects of all the isolated compounds were studied for their ability to protect PC12 cells from injury caused by oxygen-glucose deprivation and reperfusion.
Beneficial effects of endophytic fungi inoculation on tanshinones and phenolic compounds of .
Iran J Basic Med Sci
April 2023
Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran.
Objectives: is considered as a new source of tanshinone-producing plants in Iran. Symbiosis of endophytic fungi with their host plants is an effective tool to promote the growth and secondary metabolism of medicinal herbs. Therefore, using endophytic fungi as a biotic elicitor is a proper solution to increase the yield of plant products.
View Article and Find Full Text PDFLate-stage cascade of oxidation reactions during the biosynthesis of oxalicine B in .
Acta Pharm Sin B
January 2023
Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
Article Synopsis
- Oxalicine B is a complex compound with a unique structure called a bispirocyclic ring, but its biosynthesis was not well understood until this study, which focuses on key enzymes involved in its production.
- The researchers identified and characterized three important oxygenases (OxaL, OxaK, and OxaB) that play crucial roles in the biosynthetic pathway, with OxaK showing versatility in processing various intermediates.
- They also discovered new metabolites with strong anti-influenza A virus activity and provided insights into the late-stage biosynthesis of oxalicine B, suggesting potential for further enzyme-based modifications.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!