A regeneration and transformation protocol for ramie (Boehmeria nivea Gaud.) is presented. Regeneration was obtained from leaf discs placed on solid B-5 medium (Gamborg et al. 1968) containing adequate concentrations of auxin and cytokinin. Co-cultivation of leaf discs with Agrobacterium tumefaciens and subsequent regeneration resulted in transgenic plants as shown by Southern blot and analysis of expression of the GUS-marker gene.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/BF00232812 | DOI Listing |
Publication Analysis
Top Keywords
transgenic plants
8
ramie boehmeria
8
boehmeria nivea
8
nivea gaud
8
leaf discs
8
plants ramie
4
gaud agrobacterium
4
agrobacterium mediated
4
mediated transformation
4
transformation regeneration
4
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!