The squares of Ag-stained nucleolar organizing regions of metaphase chromosomes have been estimated by scanning their negative images on the film and computer data processing. The intercellular variation of the sum of squares of nucleolar organizing regiones of five individuals was studied. The coefficient of variation for these individuals varied from 11.2 to 24.6%. The analysis of the mean sum of squares of nucleolar organizing regiones per metaphase has revealed reliable differences for all the individuals. This value, therefore, can be taken as individual characteristics in the population research.

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