The extraction of functional components from radix of Arnebia euchroma was optimized using orthogonal design based on the extraction yields of shikonin, and hydroxyl-naphthoquinone pigments. The data processing was carried out with the multiple guidelines grading method for optimizing the extraction condition. Compared with the traditional method (refluxing and ultrasonic extraction), the flash extraction method was more efficient The optimal conditions were as follows: 95% ethanol extract 3 times with 90 s for each. Under these conditions, the extraction yields of shikonin, and hydroxyl-naphthoquinone pigments were 93.16%, 93.89%, respectively, and the dry extract rate was 5.16%. In conclusion, the result showed that the flash extraction technology was appropriate, stable and feasible.
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J Contemp Dent Pract
September 2024
Department of Orthodontic, Faculty of Dentistry, Mansoura University, Mansoura, Egypt.
Aim: This study evaluates long-term shear bond strength (SBS) and enamel micro cracks (MCs) healing after using adhesive pre-coated brackets (APC).
Materials And Methods: A total of eighty extracted human premolar teeth were randomly divided into four experimental groups ( = 20 per group): Control group: Teeth underwent indentation but no bracket bonding; group II : Teeth were subjected to indentation without exposure to thermocycling; group III: Teeth experienced both indentation and thermocycling; group IV: No indentation was applied to the teeth; groups III and IV were further divided into two subgroups to simulate different clinical timelines: Subgroup A (n = 10): Teeth underwent 5,000 thermocycles, equivalent to six months of clinical use. Subgroup B (n = 10): Teeth were subjected to 10,000 thermocycles, representing 12 months of use.
Molecules
December 2024
Plant Protection Institute, HUN-REN Centre for Agricultural Research, Fehérvári út 132-144, 1116 Budapest, Hungary.
Flash column chromatographic fractionation of tree of heaven () stem and trunk bark extracts, guided by thin-layer chromatography (TLC)- assay and TLC-heated electrospray high-resolution tandem mass spectrometry (HESI-HRMS/MS), lead to the isolation of six known compounds: (9,11)-13-hydroxy-9,11-octadecadienoic acid (13-HODE, ), (10,12)-9-hydroxy-10,12-octadecadienoic acid (9-HODE, ), hexadecanedioic acid (thapsic acid, ), 16-hydroxyhexadecanoic acid (juniperic acid, ), 16-feruloyloxypalmitic acid (alpinagalanate, ), and canthin-6-one (). Their structures were elucidated by HESI-HRMS/MS and one- and two-dimensional nuclear magnetic resonance (NMR) spectroscopy. This is the first study identifying - in tree.
View Article and Find Full Text PDFSci Rep
January 2025
Department of Mechanical Engineering, Adama Science and Technology University, Adama, Ethiopia.
Recycled engine oil was produced from used engine oil (15W-40) using a combination of MEK and 1-butanol solvents with an activated carbon adsorbent through the adsorption process. The experimental design matrix was prepared using Box-Behnken design (BBD) package within RSM, and recycled engine oil is optimized by response surface methodology (RSM). By varying solvent-to-oil ratio, the temperature and contact duration optimal conditions were identified as a 5.
View Article and Find Full Text PDFTurk J Orthod
December 2024
Hatay Mustafa Kemal University Faculty of Dentistry, Department of Orthodontics, Hatay, Turkey.
Objective: The aim of this study was to evaluate and compare microleakage under the conventional and flash-free ceramic brackets bonded with different agents.
Methods: Forty extracted human maxillary premolar teeth were randomly divided into five groups. According to the groups, adhesive coated and conventional bracket systems were bonded to the tooth surfaces with the specified adhesive agents.
PeerJ
December 2024
Center of Microbiome Science, Ohio State University, Columbus, Ohio, United States.
Recovered microbial community structure is known to be influenced by sample storage conditions and nucleic acid extraction methods, and the impact varies by sample type. Peat soils store a large portion of soil carbon and their microbiomes mediate climate feedbacks. Here, we tested three storage conditions and five extraction protocols on peat soils from three physicochemically distinct habitats in Stordalen Mire, Sweden, revealing significant methodological impacts on microbial (here, meaning bacteria and archaea) community structure.
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