Whilst the cytokinins are important promoters of plant cell division in vitro and in vivo, their mode of action remains unknown. Here we report the results of a study showing that a single application of a low dose of a cytokinin to the shoot apical meristem of Sinapis alba L. activates new replicon origins in chromosomal DNA, resulting in the halving of replicon size, and synchronizes the activation of replicon origins. These effects cause a 3.5-fold shortening of the duration of chromosomal DNA replication (S phase of the cell cycle). We hypothesize that one of the proteins involved in the initiation of DNA replication is a target for cytokinins.
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Article Synopsis
- The loading of MCM2-7 helicases is crucial for assembling DNA replication machinery, with human loading pathways differing from that of yeast, particularly regarding the role of ORC6.
- Through biochemical reconstitution and electron microscopy, researchers identified several intermediate states in the formation of MCM double hexamers, revealing that ORC6 enhances but is not essential for loading MCM onto DNA.
- The flexibility in the human loading process, including the potential dimerization of independently loaded MCM hexamers, may offer advantages during cellular replication stress, paving the way for future studies on DNA replication initiation.
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