Plantlets were regenerated from 5-year subcultured compact callus derived from petiole tissues of wild viola (Viola patrinii DC.) but not from 5-year subcultured friable callus. Regeneration occurred most efficiently on medium that contained two-fold diluted basal salts of Murashige and Skoog's (MS) medium, 5 × 10(-6) M 1-naphthaleneacetic acid and 10(-6) M kinetin. The effect of dilution of MS basal salts could also be achieved solely by two-fold dilution of the potassium dihydrogen phosphate in the mixture.The present study revealed that dilution of MS basal salts, in particular of potassium dihydrogen phosphate, was important for the regeneration of wild viola. Moreover, although the callus had been subcultured for 5 years, regeneration of plantlets from callus was still possible. In addition, scanning electron microscopy revealed that details of the process of plant regeneration from subcultured callus varied with the age and source of callus and differed from that reported in rice.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/BF00232919 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Stable ubiquitin conjugation for biological interrogation of ubiquitinated tau repeat domain.
Bioorg Chem
September 2024
Department of Biotechnology, University of Verona, 37134 Verona, Italy. Electronic address:
Protein semisynthesis approaches are key for gaining insights into the effects of post-translational modifications (PTMs) on the structure and function of modified proteins. Among PTMs, ubiquitination involves the conjugation of a small protein modifier to a substrate amino acid residue and is unique in controlling a variety of cellular processes. Interest has grown in understanding the role of ubiquitination in neurodegenerative conditions, including tauopathies.
View Article and Find Full Text PDFPlant production practices can influence the genetic diversity of cultivated plant materials and, ultimately, their potential to adapt to a reintroduction site. A common step in the plant production process is the application of seed pretreatment to alleviate physiological seed dormancy and successfully germinate seeds. In production settings, the seeds that germinate more rapidly may be favored in order to fill plant quotas.
View Article and Find Full Text PDFAbsorption changes in Photosystem II in the Soret band region upon the formation of the chlorophyll cation radical [PP].
Photosynth Res
December 2024
Institut de Biologie Physico-Chimique, UMR CNRS 7141 and Sorbonne Université, 13 Rue Pierre Et Marie Curie, 75005, Paris, France.
Flash-induced absorption changes in the Soret region arising from the [PP] state, the chlorophyll cation radical formed upon light excitation of Photosystem II (PSII), were measured in Mn-depleted PSII cores at pH 8.6. Under these conditions, Tyr is i) reduced before the first flash, and ii) oxidized before subsequent flashes.
View Article and Find Full Text PDFDetection is essential to studying and monitoring wild animals; however, detection is challenging for small endotherms that are nocturnal or best detected at night. Techniques such as trapping or spotlighting disturb focal species, and the effectiveness of spotlighting can be limited for cryptic species, resulting in low detection rates that hinder our ability to monitor and study some endotherms at night. Thermal scanners detect infrared wavelengths not otherwise visible to humans.
View Article and Find Full Text PDFPrevalence of in Wild Boars Hunted at Different Altitudes in the Campania and Latium Regions (Italy).
Animals (Basel)
February 2023
Department of Veterinary Medicine and Animal Production, University of Napoli-Federico II, 80137 Naples, Italy.
Recent reports of in wild boars in southern Italy have highlighted the need for collecting epidemiological data on the presence of this parasite and understanding the role of possible interactions between wild boars, , and the environment. This study analyses, using histopathological and biomolecular techniques, the presence of in tongue samples of wild boars hunted in four provinces of the Campania and Latium regions (Italy), in areas located above and below 900 m above sea level (asl). Histopathological examinations revealed the presence of adults and eggs of nematodes, which were subsequently identified as by biomolecular analysis, in the tongue epithelium.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!