The plasma-membrane H(+)-ATPase (EC 3.6.1.35) of maize (Zea mays L.) coleoptiles and enclosed leaves has been localized at the optical-microscope level utilizing paraffin sections and a specific monoclonal antibody. Both in coleoptiles and in leaves the stomatal guard cells and the phloem of vascular bundles are the tissues most enriched in ATPase. The enrichment in guard cells is relevant to active ion transport during stomata opening. Considering the postulated activation of coleoptile ATPase by auxin, it is remarkable that the ATPase is not enriched in the coleoptile epidermis, where most of the auxin receptor is located.
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Plants (Basel)
December 2024
Stage Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an 271018, China.
Under NaHCO stress, exogenous 24-epibrassinolide (EBR) markedly alleviated Na accumulation in cucumber plants, thereby decreasing the Na/K, Na/Mg, and Na/Ca ratios. This mitigation was accompanied by elevated concentrations of K, Ca, and Mg, as well as enhanced expression of the and genes. In addition, the activities of plasma membrane H-ATPase, vesicular membrane H-ATPase, and vesicular membrane H-PPase were significantly increased, contributing to the maintenance of ionic balance in cucumber plants.
View Article and Find Full Text PDFDev Cell
January 2025
School of Life Sciences, East China Normal University, Shanghai 200241, China; Institute of Eco-Chongming, East China Normal University, Shanghai 202162, China. Electronic address:
During pollen-stigma interaction, pollen coat protein B-class peptides (PCP-Bs) compete with stigmatic rapid alkalinization factor (RALF) for interaction with FERONIA/ANJEA receptor kinases (FER/ANJ), stimulating pollen hydration and germination. However, the molecular mechanism underlying PCP-Bs-induced, FER/ANJ-regulated compatible responses remains largely unknown. Through PCP-Bγ-induced phosphoproteomic analysis, we characterized a series of pollination-related signaling pathways regulated by FER/ANJ.
View Article and Find Full Text PDFPlant Biotechnol J
January 2025
Key Laboratory of Biology and Genetic Improvement of Horticultural Crops in Huang-Huai Region, Ministry of Agriculture, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai-An, China.
Here, we evaluated the role of ethylene in regulating the NaHCO stress tolerance of grapevines and clarified the mechanism by which VvERF1B regulates the response to NaHCO stress. The exogenous application of ACC and VvACS3 overexpression in grapevines and grape calli revealed that ethylene increased NaHCO stress tolerance, and this was accompanied by increased plasma membrane H-ATPase (PMA) activity. The expression of VvERF1B was strongly induced by ACC, and overexpression of this gene in grapevines conferred increased NaHCO stress tolerance and enhanced PMA activity and H and oxalate secretion.
View Article and Find Full Text PDFPlants (Basel)
December 2024
Xinjiang Key Laboratory for Ecological Adaptation and Evolution of Extreme Environment Biology, College of Life Sciences, Xinjiang Agricultural University, Urumqi 830052, China.
Plant plasma membrane (PM) H-ATPase functions as a proton-motive force by exporting cellular protons to establish a transmembrane chemical gradient of H ions and an accompanying electrical gradient. These gradients are crucial for plant growth and development and for plant responses to abiotic and biotic stresses. In this study, a comprehensive identification of the PM H-ATPase gene family was conducted across four cotton species.
View Article and Find Full Text PDFNat Commun
January 2025
School of Infection and Immunity, University of Glasgow, Sir Graeme Davies Building, 120 University Place, Glasgow, G12 8TA, UK.
For the protozoan parasite Leishmania, completion of its life cycle requires sequential adaptation of cellular physiology and nutrient scavenging mechanisms to the different environments of a sand fly alimentary tract and the acidic mammalian host cell phagolysosome. Transmembrane transporters are the gatekeepers of intracellular environments, controlling the flux of solutes and ions across membranes. To discover which transporters are vital for survival as intracellular amastigote forms, we carried out a systematic loss-of-function screen of the L.
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