AI Article Synopsis
- A coumarin-based agent used for photolabeling showed a significant increase in fluorescence after it bonded with a target protein.
- The diazirine part of the agent initially reduced the fluorescence emitted by the coumarin segment.
- When exposed to 365 nm light after photolysis treatment, the fluorescence was dramatically restored.
Article Abstract
A coumarin-fused diazirine photolabeling agent exhibited dramatic enhancement in fluorescence after cross-linking with the target protein. Fluorescence emission from the coumarin moiety was efficiently quenched by the diazirine group, and was then intensively recovered from photolysis treatment by irradiation with light at a wavelength of 365 nm.
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| http://dx.doi.org/10.1039/c3cc45780j | DOI Listing |
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