New antimicrobial properties of products derived from Humulus lupulus L. such as antiadherent and antibiofilm activities were evaluated. The growth of gram-positive but not gram-negative bacteria was inhibited to different extents by these compounds. An extract of hop cones containing 51% xanthohumol was slightly less active against S. aureus strains (MIC range 31.2-125.0 μg/mL) than pure xanthohumol (MIC range 15.6-62.5 μg/mL). The spent hop extract, free of xanthohumol, exhibited lower but still relevant activity (MIC range 1-2 mg/mL). There were positive coactions of hop cone, spent hop extracts, and xanthohumol with oxacillin against MSSA and with linezolid against MSSA and MRSA. Plant compounds in the culture medium at sub-MIC concentrations decreased the adhesion of Staphylococci to abiotic surfaces, which in turn caused inhibition of biofilm formation. The rate of mature biofilm eradication by these products was significant. The spent hop extract at MIC reduced biofilm viability by 42.8%, the hop cone extract by 74.8%, and pure xanthohumol by 86.5%. When the hop cone extract or xanthohumol concentration was increased, almost complete biofilm eradication was achieved (97-99%). This study reveals the potent antibiofilm activity of hop-derived compounds for the first time.
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http://dx.doi.org/10.1155/2013/101089 | DOI Listing |
Chem Biodivers
December 2024
M'sila University, Department of Natural and Life Sciences, Road Bordj Bou Arreiridj, M'sila 28000 Algeria, M'sila, ALGERIA.
The study examines the chemical composition and antimicrobial properties of petroleum ether and hydro-methanolic extracts of Achillea santolina(A. santolina) from Algeria. Chemical profiling was performed using High-Performance Liquid Chromatography with Diode Array Detection (HPLC-DAD) for the hydro-methanolic extract and Gas Chromatography-Mass Spectrometry (GC-MS) for the petroleum ether extract.
View Article and Find Full Text PDFAppl Environ Microbiol
December 2024
Department of Microbiology, Biochemistry, & Molecular Genetics, Rutgers New Jersey Medical School, Newark, New Jersey, USA.
Because of the urgent need for new antibiotics to treat drug-resistant bacterial pathogens, we employed an assay that rapidly screens large quantities of compounds for their ability to interfere with bacterial protein synthesis, in particular, the delivery of amino acids to the ribosome via tRNA and elongation factor Tu (EF-Tu). We have identified a drug lead, named MGC-10, which kills Gram-positive bacteria, including methicillin-resistant (MRSA), with a MIC of 6 µM, while being harmless to mammalian cells in that concentration range. The antibacterial activity of MGC-10 was broad against over 50 strains of antibiotic-resistant samples obtained from hospital infections, where MGC-10 inhibited all tested strains of MRSA.
View Article and Find Full Text PDFFront Plant Sci
December 2024
Department of Biochemistry, Faculty of Pharmacy, Harran University, Sanliurfa, Türkiye.
In this study, L. extracts were obtained using various green extraction techniques, including supercritical CO, subcritical ethanol, and ultrasound-assisted extraction, each performed under optimized parameters. The phytochemical content of the extracts was analyzed using the LC-MS/MS technique, quantifying 53 phytochemicals.
View Article and Find Full Text PDFBioorg Med Chem
December 2024
Key Laboratory of Medical Laboratory Diagnostics of the Education Ministry, College of Laboratory Medicine, Chongqing Medical University, No. 1, Yixueyuan Road, Yuzhong Dist, Chongqing 400016, China. Electronic address:
Acetohydroxy acid synthase (AHAS) is a key enzyme that catalyzes the synthesis of branched-chain amino acids, which is indispensable for the survival and growth of Mycobacterium tuberculosis (Mtb). Aim to discover new AHAS inhibitors from natural products, here we performed computer assistant target-based screening for Mtb-AHAS inhibitors using Discovery Studio on TCMSP and SELLECK libraries. Mtb-AHAS structure was first simulated and verified for docking, and 80 compounds with top LIBDOCK and CDDOCK scores were obtained.
View Article and Find Full Text PDFJ Microbiol Methods
December 2024
Medical Microbiology, Division of Infection and Immunity, Cardiff University, Cardiff, UK; UK Health Security Agency, Bacteriology Reference Department, London, UK. Electronic address:
Currently there is no detailed, internationally agreed protocol defined to evaluate antimicrobial susceptibility testing (AST) for Legionella pneumophila (required to establish epidemiological cut-off value or "ECOFF" boundaries); therefore, antimicrobial resistance in these isolates cannot be defined. AST methods utilising media containing activated charcoal as an ingredient, to enable Legionella growth, are unreliable as noted in an internationally authored opinion paper and a new gold standard is required. Here we define a detailed protocol for broth microdilution (BMD) using defined cell culture collection-deposited control reference strains (Philadelphia-1 and Knoxville-1) as well as two accessible reference strains with moderately (lpeAB-carrying) and markedly (23S rRNA mutation-carrying) elevated azithromycin minimum inhibitory concentration (MIC).
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