AI Article Synopsis
- Researchers successfully recovered transgenic rice plants by introducing plasmids with selectable and screenable marker genes into elite rice varieties using electric-discharge particle acceleration.
- Analysis showed a 100% co-integration frequency, with most plants containing 1 to 10 copies of the introduced DNA, which were inherited as a single dominant hemizygous locus.
- Co-expression of unselected genes varied, reaching up to 90% for certain constructs, and detailed analysis was conducted on the integrative structures of unique transgenic loci in rare R0 plants.
Article Abstract
The recovery of transgenic rice plants expressing a number of exogenous genes was reported previously. Using immature embryo explants as the target tissue, plasmids containing both selectable and screenable marker genes were introduced into elite rice varieties via electric-discharge particle acceleration. Co-integration, copy number, expression, and inheritance of these genes were analyzed. A 100% co-integration frequency was confirmed by Southern-blot analyses of R0 plants. The majority of transgenic plants contained between one and ten copies of exogenous DNA and molecular and genetic analyses of progeny indicated that all copies in almost all R0 plants were inherited as a single dominant hemizygous locus. Co-expression of unselected genes ranged from 30-66% for gus/hmr constructs, depending on the promotor used, and up to 90% for bar/hmr constructs. The integrative structures of two unlinked transgenic loci of a rare R0 plant were analyzed in detail by Southern-blot analysis of its progeny.
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