AI Article Synopsis
- The study aimed to detect antibodies against lens βH-crystallins in the serum of American Cocker Spaniels, focusing on those with and without cataracts and uveitis.
- A total of 73 American Cocker Spaniels and 6 normal Beagles were involved, with serum samples analyzed using electrophoresis and immunoblots.
- Results indicated that while many ACS had antibodies to βH-crystallins, there was no clear link to cataract stages, although specific antibodies to βA1-crystallin were found only in cataract patients, suggesting a potential connection to cataract development.
Article Abstract
Objective: To detect antibodies for lens βH-crystallins in the serum from the American Cocker Spaniel (ACS) presenting with and without cataracts and with and without uveitis.
Animal Studied: Seventy-three American Cocker Spaniels and six normal Beagles.
Procedures: Sera were collected from 73 ACSs, including those with normal lenses and those with cataracts, or uveitis. Fractionated, normal Beagle lens βH-crystallins were separated by one- or two-dimensional electrophoresis. The separated lens βH-crystallins were used on immunoblots as sentinel substrates against which the ACS sera were tested for the presence of antibodies against βH-crystallins.
Results: Sera from approximately two-thirds of study animals contained antibodies to some βH-crystallin polypeptides, but reactivity varied among patients. Contrary to some hypotheses, serum antibodies to groups of βH-crystallins did not relate to the stages of cataract. However, detailed analysis by two-dimensional immunoblotting and mass spectrometry showed that three spots originating from βA1-crystallin were detected only in sera from cataract patients.
Conclusion: Serum antibodies to βA1-crystallin may be associated with the development of cataract.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4371642 | PMC |
| http://dx.doi.org/10.1111/vop.12113 | DOI Listing |
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