AI Article Synopsis
- An effective cancer therapy should specifically target tumors while minimizing side effects.
- Researchers engineered a weakened strain of Salmonella typhimurium to deliver a gene called Apoptin into human cancer cells, showing increased cell death through a specific cellular pathway.
- Tests in mice demonstrated that this recombinant bacteria slowed tumor growth and improved survival rates, suggesting its promise as a cancer treatment.
Article Abstract
An effective cancer therapeutic should target tumours specifically with limited systemic toxicity. Here, we transformed an attenuated Salmonella typhimurium (S. typhimurium) with an Apoptin expressing plasmid into a human laryngeal carcinoma cell line. The expression of the inserted gene was measured using fluorescence and immunoblotting assays. The attenuated S. typhimurium-mediated Apoptin significantly decreased cytotoxicity and strongly increased cell apoptosis through the activation of caspase-3. The process was mediated by Bax, cytochrome c and caspase-9. A syngeneic nude murine tumour model was used to determine the anti-tumour effects of the recombinant bacteria in vivo. Systemic injection of the recombinant bacteria with and without re-dosing caused significant tumour growth delay and reduced tumour microvessel density, thereby extending host survival. Our findings indicated that the use of recombinant Salmonella typhimurium as an Apoptin expression vector has potential cancer therapeutic benefits.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3805922 | PMC |
| http://dx.doi.org/10.7150/ijms.6960 | DOI Listing |
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