Graphene is a novel carbonic material with great potentials for the use as sorbent due to its ultrahigh surface area. Herein, we report the use of graphene as sorbent in solid-phase extraction (SPE) using pipette tip as cartridge namely GPT-SPE, together with ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS), for the analysis of lipophilic marine toxins (LMTs), including yessotoxins (YTX), okadaic acid (OA), dinophysistoxin-1 (DTX1), gymnodimine (GYM), spirolides-1 (SPX1), pectenotoxin-2 (PTX2) and azaspiracid-1 (AZA1) in shellfish. The GPT-SPE procedure was optimized and the performance of graphene was fully validated. Results with high-sensitivity and good reproducibility was obtained and compared with that of other sorbents like C18 silica, multi-walled carbon nanotubes (MWCNTs), commercial Oasis HLB, and Strata-X for the extraction of LMTs, which showed superiority and advantages of graphene, such as good recoveries, stability and compatibility with various solvents. In order to exhibit the potentials of graphene as an excellent sorbent material, 67 mussel samples from six coastal cities of China were analyzed. OA was found to be the dominant contaminant, while YTX was also detected with low level.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.talanta.2013.07.042 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Time dependence of the pre-chromatographic oxidation method for bivalves contaminated with paralytic shellfish poisoning toxins.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess
March 2025
Sea and Marine Resources Department (DMRM), The Portuguese Sea and Atmosphere Institute, I.P. (IPMA, IP), Algés, Portugal.
The neurotoxins of the saxitoxin family can be the origin of the human neurological syndrome of paralytic shellfish poisoning via contaminated marine bivalve vectors. A pre-chromatographic oxidation method is the official testing method in the EU, also known as the 'Lawrence method'. It involves several steps, including toxin extraction, solid-phase clean-up, and oxidation to produce fluorescent derivatives.
View Article and Find Full Text PDFThe proteotranscriptomic characterization of venom in the white seafan elucidates the evolution of Octocorallia arsenal.
Open Biol
March 2025
IBMM, University of Montpellier, CNRS, ENSCM, Montpellier, France.
All the members of the phylum Cnidaria are characterized by the production of venom in specialized structures, the nematocysts. Venom of jellyfish (Medusozoa) and sea anemones (Anthozoa) has been investigated since the 1970s, revealing a remarkable molecular diversity. Specifically, sea anemones harbour a rich repertoire of neurotoxic peptides, some of which have been developed in drug leads.
View Article and Find Full Text PDFBlue-lined octopus Hapalochlaena fasciata males envenomate females to facilitate copulation.
Curr Biol
March 2025
Queensland Brain Institute, The University of Queensland, St Lucia, QLD 4072, Australia; School of the Environment, The University of Queensland, St Lucia, QLD 4072, Australia.
A variety of phylogenetically distant taxa, including flatworms, mollusks, amphibians, and fishes, use the deadly neurotoxin tetrodotoxin (TTX) for predation and defense. A well-known example is the blue-lined octopus, Hapalochlaena fasciata (Hoyle, 1886), which uses symbiotic bacteria to sequester TTX in its posterior salivary glands (PSG). When it bites, the TTX-laden saliva immobilizes large prey and has caused lethal envenomation in a few incidents involving humans.
View Article and Find Full Text PDFCryptic diversity within the Gonyaulax spinifera species complex, its relation to the cyst-defined species Spiniferites bentorii, S. mirabilis and S. membranaceus, with the description of Gonyaulax carbonell-mooreae sp. nov. (Gonyaulacales, Dinophyceae).
J Phycol
March 2025
Third Institute of Oceanography, Ministry of Natural Resources, Xiamen, China.
The fossil dinoflagellates Spiniferites bentorii, S. mirabilis, and S. membranaceus are known to inhabit recent sediments and are often used to reconstruct past sea-surface conditions.
View Article and Find Full Text PDFA noncanonical intrinsic terminator in the HicAB toxin-antitoxin operon promotes the transmission of conjugative antibiotic resistance plasmids.
Nucleic Acids Res
February 2025
Key Laboratory of Tropical Oceanography, Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, No.1119, Haibin Road, Nansha District, Guangzhou 511458, China.
Conjugative plasmids, major vehicles for the spread of antibiotic resistance genes, often contain multiple toxin-antitoxin (TA) systems. However, the physiological functions of TA systems remain obscure. By studying two TA families commonly found on colistin-resistant IncI2 mcr-1-bearing plasmids, we discovered that the HicAB TA, rather than the StbDE TA, acts as a crucial addiction module to increase horizontal plasmid-plasmid competition.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!