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A study of extraction process and in vitro antioxidant activity of total phenols from Rhizoma Imperatae. | LitMetric

A study of extraction process and in vitro antioxidant activity of total phenols from Rhizoma Imperatae.

Afr J Tradit Complement Altern Med

College of Life Science and Technology, Yangtze Normal University, Fuling 408100, P. R. China.

Published: May 2014

AI Article Synopsis

  • The study focused on the extraction method of Rhizoma Imperatae and evaluated its antioxidant potential to support its effective use.
  • An optimal extraction process was identified, using water at 30 °C across three 2-hour extraction cycles, leading to a significant yield of antioxidant compounds.
  • Results indicated that the extract has strong hydroxyl radical scavenging ability and reducing power comparable to ascorbic acid, highlighting its high polyphenol content and antioxidative properties.

Article Abstract

The study investigated the extraction method of Rhizoma Imperatae and its antioxidant activity, and provided a basis for its rational development. The extraction method of Rhizoma Imperatae was determined using orthogonal design test and by total phenol content, its hydroxyl radical scavenging ability was measured by Fenton reaction, and potassium ferricyanide reduction method was used to determine its reducing power. The results showed that the optimum extraction process of Rhizoma Imperatae was a 50-fold volume of water, 30 °C, three times of extraction with 2 h each. Its IC50 for scavenging of hydroxyl radicals was 0.0948 mg/mL, while IC50 of ascorbic acid was 0.1096 mg/mL; in the ferricyanide considerable reduction method, the extract exhibited reducing power comparable to that of the ascorbic acid. The study concluded that Rhizoma Imperatae extract contains relatively large amount of polyphenols, and has a good anti-oxidation ability.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3794410PMC
http://dx.doi.org/10.4314/ajtcam.v10i4.28DOI Listing

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