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Investigation of the threonine metabolism of Echinococcus multilocularis: The threonine dehydrogenase as a potential drug target in alveolar echinococcosis.
Int J Parasitol Drugs Drug Resist
January 2025
Institute of Parasitology, Vetsuisse Faculty, University of Bern, Bern, Switzerland; Multidisciplinary Center for Infectious Diseases, University of Bern, Bern, Switzerland. Electronic address:
Alveolar echinococcosis (AE) is a severe zoonotic disease caused by the metacestode stage of the fox tapeworm Echinococcus multilocularis. We recently showed that E. multilocularis metacestode vesicles scavenge large amounts of L-threonine from the culture medium.
View Article and Find Full Text PDFInteractions of metal-based nanozymes with aptamers, from the design of nanozyme to its application in aptasensor: Advances and perspectives.
Talanta
December 2024
Chongqing Key Laboratory of Conservation and Utilization of Freshwater Fishes, Animal Biology Key Laboratory of Chongqing Education Commission of China, Chongqing Normal University, College of Life Sciences, Chongqing, 401331, China. Electronic address:
Nanozymes, characterized by enzyme-like activity, have been extensively used in quantitative analysis and rapid detection due to their small size, batch fabrication, and ease of modification. Researchers have combined aptamers, an emerging molecular probe, with nanozymes for biosensing to address the limited reaction specificity of nanozymes. Nanozyme aptasensors are currently experiencing significant growth, offering a promising solution to the lack of rapid detection methods across various fields.
View Article and Find Full Text PDFEngineering for production of active cannabinoid synthases via secretory pathway optimization.
Biotechnol Rep (Amst)
March 2025
Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
The production of cannabinoid compounds such as Δ9-tetrahydrocannabinol (THC), cannabidiol (CBD) and cannabichromene (CBC) with potential pharmaceutical applications is growing sharply. However, challenges such as the low yield of minor cannabinoids, legal restrictions on cultivation, and the complexity and cost of purification from the Cannabis sativa plant necessitate a biotechnological approach. Since the biosynthetic pathway is disclosed, cannabinoids have been produced in yeast, insect cells and plants mainly by the heterologous expression of tetrahydrocannabinol acid synthase (THCAS).
View Article and Find Full Text PDFIdentification and functional analysis of novel homozygous LMF1 variants in severe hypertriglyceridemia.
J Clin Lipidol
October 2024
Department of Biomedical Sciences, Western University of Health Sciences, Pomona, CA, USA. Electronic address:
Background: The genetic basis of hypertriglyceridemia (HTG) is complex and includes variants in Lipase Maturation Factor 1 (LMF1), an endoplasmic reticulum (ER)-chaperone involved in the post-translational activation of lipoprotein lipase (LPL).
Objective: The objective of this study was to identify and functionally characterize biallelic LMF1 variants in patients with HTG.
Methods: Genomic DNA sequencing was used to identify biallelic LMF1 variants in HTG patients without deleterious variants in LPL, apolipoprotein C-II (APOC2), glycosylphosphatidylinositol-anchored high-density lipoprotein binding protein 1 (GPIHBP1) or apolipoprotein A-V (APOA5).
Purification of Enzymatically Active Xrn1 for Removal of Non-capped mRNAs from In Vitro Transcription Reactions and Evaluation of mRNA Decapping Status In Vivo.
Methods Mol Biol
November 2024
Laboratory of RNA Processing and Decay, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland.
Article Synopsis
- The cap structure on mRNA, specifically the 7-methylguanosine, is crucial for stability and translation initiation in eukaryotic cells.
- Research has focused on understanding mRNA decay mechanisms, particularly the process of decapping, which is key to mRNA lifespan and translation efficiency.
- Recent advancements in RNA-based vaccines highlight the need for effective methods to eliminate non-capped mRNAs, and this text presents a protocol for producing and purifying the enzyme Xrn1, which is vital for this purpose.
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