AI Article Synopsis
- The study focused on the replication patterns of CHO and HeLa cells after synchronization at the end of the G1 phase, using pulse labeling every hour.
- The results indicated distinct R-type replication patterns in early S-phase and a shift to G- and C-band replication in late S-phase, signifying a sudden transition in chromatin replication.
- The paper also discusses the differences observed between these cell lines and previous studies on other hamster and human cell types.
Article Abstract
Chinese hamster ovary (CHO) and HeLa cells were successively pulse labelled at 1-h intervals after the cultures were synchronized at the end of G1 (monitored by flow cytometry). The metaphases analysed afterwards showed R-type replication patterns after 1-h pulses during the early S-phase (SE; from h 1-5 after release) and replication of G- and C-bands in late S-phase (SL: from h 6-8 after release). The transition from SE to SL is abrupt, constituting a sudden switch of replication between different types of chromatin as has been described for human lymphocytes. The differences between these two cell lines and earlier results reported on a V79 Chinese hamster cell line and on normal diploid human and Chinese hamster fibroblasts are discussed.
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| http://dx.doi.org/10.1007/BF00327468 | DOI Listing |
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