AI Article Synopsis
- The study focuses on using a 2-Dimensional Electrophoresis (2-DE) approach to analyze how plants, specifically carnations, respond to the pathogenic fungus Fusarium oxysporum.
- The research emphasizes the importance of optimizing proteomics protocols to fit the unique biological systems and research goals.
- The findings on protein extraction and separation techniques can be applied to other plant-pathogen interactions to assess how pathogens affect the host's protein profile and to identify key proteins involved in early plant defense responses.
Article Abstract
We are currently using a 2-DE-based proteomics approach to study plant responses to pathogenic fungi by using the carnation (Dianthus caryophyllus L)-Fusarium oxysporum f. sp. dianthi pathosystem. It is clear that the protocols for the first stages of a standard proteomics workflow must be optimized to each biological system and objectives of the research. The optimization procedure for the extraction and separation of proteins by 1-DE and 2-DE in the indicated system is reported. This strategy can be extrapolated to other plant-pathogen interaction systems in order to perform an evaluation of the changes in the host protein profile caused by the pathogen and to identify proteins which, at early stages, are involved or implicated in the plant defense response.
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| http://dx.doi.org/10.1007/978-1-62703-631-3_39 | DOI Listing |
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