Objective: To establish an identification method of Cudrania tricuspidata and Cudrania cochinchinensis, and their medicinal parts, and analyse the species of commercial drug Cudraniae Tricuspidatae Radix et Caulis.

Methods: TLC and First-order Derivative UV Spectrophotometry were used.

Results: Commercial drugs 5, 6, 7 and 9 belonged to the stem of Cudrania cochinchinensis, commercial drug 1, 2, 3 ,4 and 8 were neither Cudrania tricuspidata nor Cudrania cochinchinensis.

Conclusion: Cudrania tricuspidata and Cudrania cochinchinensis can be identified by TLC, as well as the medicinal parts. UV Spectrophotometry can't be applied to variety identification but can be used for identifying the medicinal parts. It's more accurate and reliable for identifying Cudrania tricuspidata and Cudrania cochinchinensis, and the medicinal parts by combined using TLC and UV. This study provides a scientific and effective method for species identification and medicinal part analysis of commercial drug Cudraniae Tricuspidatae Radix et Caulis.

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