Objective: To provide a reference evidence for choosing salt-resistent and drought-resistent varieties of Isatis indigotica Fort..
Methods: The tissue culture seedlings of different Isatis indigotica tetraploids were used as plant materials. The diploid was used as CK, which were cultured for 30 days. 2 g/L NaCl and 15% PEG-6000 were used as stress treatment respectively for 12 hours, then poured out the solution, the materials were continuously cultured for 7 days. The contents of indirubin and indigotin were determined by HPLC.
Results: The contents of indigotin and indirubin of DB3, DB5 and DB12 with PEG treatment and DB2, DB3 and DB12 with NaCl treatment were increased.
Conclusion: DB3 and DB12 can be further studied as reproduction materials.
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