AI Article Synopsis
- Macrophages isolated from malignant effusions were cultured in a medium enriched with granulocyte-macrophage colony-stimulating factor (CSF) to study their growth and functionality.
- The macrophage population increased sixfold over 15 days in culture, and they retained specific cell surface markers for up to five weeks.
- These cultured macrophages demonstrated important functions such as phagocytosis and secretion of lysozyme, suggesting that human tumor-derived macrophages can be effectively expanded in a short-term culture environment enriched with GM CSF.
Article Abstract
Macrophages, isolated from malignant effusions, were cultured in vitro in media containing GCT/CM as a source of granulocyte-macrophage (GM) colony-stimulating factor (CSF). Cells were periodically examined for macrophage-specific cell surface antigens and functional activity. The number of macrophages increased sixfold during 15 days in culture. Autoradiographic studies confirmed that macrophages were incorporating 3H-Tdr. Macrophage-specific cell surface markers were retained up to five weeks in culture. Cells were also able to express macrophage-specific functions including phagocytosis and secretion of lysozyme. The results indicate that human tumor-derived macrophages may be expanded in short-term culture in the presence of GM CSF.
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