AI Article Synopsis
- The study focused on isolating carcinoembryonic antigen (CEA) from colon cancer metastases and deglycosylating it to obtain a purified protein fraction known as PF CEA.
- Research involved creating monospecific antiserum to PF CEA and comparing its properties with CEA and a non-specific antigen, NCA-1, using these antisera.
- Results indicated that certain key regions of CEA, which play a crucial role in immune response, depend on the specific three-dimensional shape of the molecule, influenced by its sugar components.
Article Abstract
Carcinoembryonic antigen (CEA) isolated from metastases of colon adenocarcinoma was subjected to deglycosylation with liquid hydrogen fluoride. The protein fraction obtained (PF CEA) was used for to prepare monospecific antiserum to PF CEA. Comparative studies of CEA, PF CEA, and non-specific cross-reacting antigen (NCA-1) have been carried out using monospecific antisera. Circular dichroism spectra of CEA and PF CEA have been studied. The data obtained suggest that some immunodominant regions of CEA are topographic, and their formation needs a specific conformation of the macromolecule, which is stabilized by the sugar moiety.
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