AI Article Synopsis
- The study successfully identified genes for the alpha and beta subunits of T-cell receptors using subtractive cDNA cloning and partial protein sequencing.
- The research discovered a gamma chain gene in a subtractive cDNA library from a specific cytotoxic T lymphocyte clone, showing similarities to the alpha and beta genes regarding structure and expression.
- Evidence indicates that while alpha and beta genes are equally expressed in both cytotoxic T (Tc) cells and helper T (TH) cells, gamma gene expression is not necessary for TH cells.
Article Abstract
Subtractive complementary DNA cloning combined with partial protein sequencing has allowed identification of the genes encoding the alpha and beta subunits of T-cell receptors. The subtractive cDNA library prepared from the cytotoxic T lymphocyte (Tc) clone 2C has been found to contain a third type of clone encoding the gamma chain. The gamma gene shares several features with the alpha and beta genes: (1) assembly from gene segments resembling immunoglobulin V, J and C (respectively variable, joining and constant region) DNA segments; (2) rearrangement and expression in T cells and not in B cells; (3) sequences reminiscent of transmembrane and intracytoplasmic regions of integral membrane proteins; (4) a cysteine residue at the position expected for an interchain disulphide bond. The alpha and beta genes are expressed at equivalent levels in both Tc cells and helper T cells (TH). The gamma gene, obtained from 2C, has been found to be expressed in all Tc cells studied. Here we present evidence that strongly suggests that TH cells do not require gamma gene expression.
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| http://dx.doi.org/10.1038/317068a0 | DOI Listing |
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