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In vivo immune cell distribution of gold nanoparticles in naïve and tumor bearing mice. | LitMetric

AI Article Synopsis

  • Gold nanoparticles (AuNPs) show potential for drug delivery and cancer immunotherapy, but their distribution within immune cells, especially in the spleen, had not been thoroughly studied.
  • Flow cytometry revealed that around 30% of the AuNPs were taken up by B cells, with smaller percentages in other immune cells such as granulocytes, dendritic cells, T cells, and myeloid-derived suppressor cells.
  • The study suggests that AuNPs are carried by immune cells as they migrate through the spleen, and particle distribution remains consistent regardless of whether the mice were naïve or tumor-bearing, highlighting implications for future nanoparticle design in immunotherapy.

Article Abstract

Gold nanoparticles (AuNP) have been widely used for drug delivery and have recently been explored for applications in cancer immunotherapy. Although AuNPs are known to accumulate heavily in the spleen, the particle distribution within immune cells has not been thoroughly studied. Here, cellular distribution of Cy5 labeled 50 nm AuNPs is characterized within the immune populations of the spleen from naïve and tumor bearing mice using flow cytometry. Surprisingly, approximately 30% of the detected AuNPs are taken up by B cells at 24 h, with about 10% in granulocytes, 18% in dendritic cells, and 8% in T cells. In addition, 3% of the particles are detected within myeloid derived suppressor cells, an immune suppressive population that could be targeted for cancer immunotherapy. Furthermore, it is observed that, over time, the particles traveled from the red pulp and marginal zone to the follicles of the spleen. Taking into consideration that the particle cellular distribution does not change at 1, 6 and 24 h, it is highly suggestive that the immune populations carry the particles and migrate through the spleen instead of the particles migrating through the tissue by cell-cell transfer. Finally, no difference is observed in particle distribution between naïve and tumor bearing mice in the spleen, and nanoparticles are detected within 0.7% of dendritic cells of the tumor microenvironment. Overall, these results can help inform and influence future AuNP delivery design criteria including future applications for nanoparticle-mediated immunotherapy.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3962812PMC
http://dx.doi.org/10.1002/smll.201301998DOI Listing

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