Biorepositories are collections of surgically obtained human tissues for current and future investigations of disease mechanisms, therapeutics, and diagnostics. In ophthalmology, a critical challenge is how to interface the operating room with the laboratory. To attain standards required for basic research, clinical and research teams must cooperate to collect, annotate, and store specimens that yield consistent results required for advanced molecular techniques. We developed an efficient platform for obtaining vitreous and other eye tissues from the operating room and transferring them to the lab. The platform includes a mobile lab cart for on-site tissue processing, a multi-user, web-based database for point-of-care phenotypic capture, and an integrated data tracking system for long-term storage. These biorepository instruments have proven essential for our studies in ophthalmic disease proteomics. This system can be implemented in other operating rooms and laboratories for a variety of biological tissues.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3964151 | PMC |
| http://dx.doi.org/10.1002/prca.201300043 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Combining Transversus Abdominis Plane and Rectus Sheath Blocks in Open Inguinal Hernia Surgery Anesthesia: A Retrospective Cohort Analysis.
World J Surg
January 2025
Department of Gastroenterological Surgery, Tampere University Hospital, Tampere, Finland.
Background: Lichtenstein hernia repair is a common surgical procedure. Previously, combined rectus sheath (RS) and transversus abdominis plane (TAP) blocks have been shown to be beneficial in laparoscopic inguinal hernia surgery. Our hypothesis is that combining the two blocks will also be beneficial in open Lichtenstein hernioplasty day-case procedures.
View Article and Find Full Text PDFHigh-Performance Hydrogen Sensing at Room Temperature via Nb-Doped Titanium Oxide Thin Films Fabricated by Micro-Arc Oxidation.
Nanomaterials (Basel)
January 2025
Guangdong Key Laboratory of Materials and Equipment in Harsh Marine Environment, School of Ocean Engineering, Guangzhou Maritime University, Guangzhou 510725, China.
Metal oxide semiconductor (MOS) hydrogen sensors offer advantages, such as high sensitivity and fast response, but their challenges remain in achieving low-cost fabrication and stable operation at room temperature. This study investigates Nb-doped TiO (NTO) thin films prepared via a one-step micro-arc oxidation (MAO) with the addition of NbO nanoparticles into the electrolyte for room-temperature hydrogen sensing. The characterization results revealed that the incorporation of NbO altered the film's morphology and phase composition, increasing the Nb content and forming a homogeneous composite thin film.
View Article and Find Full Text PDFConsensus-Based Guidelines for Best Practices in the Selection and Use of Examination Gloves in Healthcare Settings.
Nurs Rep
January 2025
Escola Enfermagem (Porto), Faculdade Ciências da Saúde e Enfermagem, Universidade Católica Portuguesa, 4169-005 Porto, Portugal.
Background/objectives: Healthcare-associated infections (HAIs) and antimicrobial resistance (AMR) present significant challenges in modern healthcare, leading to increased morbidity, mortality, and healthcare costs. Examination gloves play a critical role in infection prevention by serving as a barrier to reduce the risk of cross-contamination between healthcare workers and patients. This manuscript aims to provide consensus-based guidelines for the optimal selection, use, and disposal of examination gloves in healthcare settings, addressing both infection prevention and environmental sustainability.
View Article and Find Full Text PDFIs Membrane Filtration Applicable for the Recovery of Biologically Active Substances from Spent Lavender?
Membranes (Basel)
January 2025
Department of Chemical Engineering, University of Chemical Technology and Metallurgy, 1576 Sofia, Bulgaria.
This study explored the batch membrane filtration of 40% ethanol extracts from spent lavender, containing valuable compounds like rosmarinic acid, caffeic acid, and luteolin, using a polyamide-urea thin film composite X201 membrane. Conducted at room temperature and 20 bar transmembrane pressure, the process demonstrated high efficiency, with rejection rates exceeding 98% for global antioxidant activity and 93-100% for absolute concentrations of the target components. During concentration, the permeate flux declined from 2.
View Article and Find Full Text PDFA Systematic Review and Meta-Analysis of Prophylactic Vasopressors for the Prevention of Peri-Intubation Hypotension.
Diseases
December 2024
Department of Pulmonary and Critical Care, Mayo Clinic, Jacksonville, FL 32224, USA.
Background/objectives: Peri-intubation hypotension is a known complication of endotracheal intubation. In the hospital setting, peri-intubation hypotension has been shown to increase hospital mortality and length of stay. The use of prophylactic vasopressors at the time of sedation induction to prevent peri-intubation hypotension has been raised.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!