To investigate the relation between cell-substratum adhesion and cell-spreading we have isolated variants of anchorage-independent cells which fail to adhere to fibronectin. The variants are poorly adhesive both to fibronectin and serum, show dramatically altered morphology in culture and are unable to spread on any protein-coated surface yet tested.
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Contribution of the intracellular domain of murine Fc-gamma receptor type IIB1 to its tumor-enhancing potential.
Int J Cancer
October 1996
Department of Cell Research and Immunology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Israel.
We have previously shown that Fc gamma receptor type II B1 (Fc(gamma)RIIB1), when expressed on non-lymphoid tumor cells, significantly enhanced their tumorigenic phenotype. This study elucidates the role of the intracellular domain of Fc(gamma)RIIB1 in the enhancement of the malignant phenotype of polyoma-transformed 3T3 cells. We investigated the tumorigenic potential conferred by different variants of the receptor: Fc(gamma)RIIB1, a full-length receptor (B1) whose intracellular region is encoded by exons 8, 9 and 10; Fc(gamma)RIIB2, a spliced variant (B2) whose cytoplasmic domain comprises exons 9 and 10 and lacks exon 8; and Fc(gamma)RIIB1-CT53, a deleted mutant whose cytoplasmic domain contains the fragment encoded by exon 8 alone.
View Article and Find Full Text PDFSome cellular and molecular characteristics of high and low tumorigenicity variants of polyoma-virus transformed cells.
Mol Immunol
December 1990
Department of Microbiology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Israel.
We analyzed several cellular and molecular properties of BALB/c 3T3 cellular clones transformed in vitro with polyoma virus and exhibiting a high or low tumorigenicity phenotype. We also analyzed the same clones after a single in vivo passage in syngeneic mice. This passage invariably induced and/or selected variants exhibiting a very high tumorigenicity phenotype.
View Article and Find Full Text PDFVariants of polyoma-transformed BHK21 cells unresponsive to fibronectin.
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To investigate the relation between cell-substratum adhesion and cell-spreading we have isolated variants of anchorage-independent cells which fail to adhere to fibronectin. The variants are poorly adhesive both to fibronectin and serum, show dramatically altered morphology in culture and are unable to spread on any protein-coated surface yet tested.
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An untransformed parental Chinese hamster cell line, one polyoma transformed clone, and two clones containing both SV40 and polyoma DNA were tested for the appearance of variants resistant to 6-thioguanine or oubain. The frequency of such variants was found to be highest in doubly transformed cells. The mutation rate at these loci was correlated with the level of transformation.
View Article and Find Full Text PDFUsing a series of cold-sensitive variants of chemically transformed BHK-21 cells, revertants to the normal phenotype derived from a dimethyl-nitrosamine transformed clone of BHK-21 as well as revertants to the normal phenotype derived from polyoma transformed BHK-21 cells we have demonstrated that the surface phenotype described by enhanced agglutinability with Con A and WGA can be dissociated from the transformed phenotype described by anchorage independence (growth in semisolid medium). Specifically we have demonstrated that the surface characteristic of enhanced agglutinability may be found in a variety of cell lines which fail to display to grow in agar. Our work clearly shows that the two phenotypes described are not concomitantly controlled and tends to suggest that the phenotype of enhanced lectin agglutinability may be dissociated from the transformed phenotype.
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