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Web-based visualisation and analysis of 3D electron-microscopy data from EMDB and PDB. | LitMetric

Web-based visualisation and analysis of 3D electron-microscopy data from EMDB and PDB.

J Struct Biol

Protein Data Bank in Europe, European Molecular Biology Laboratory, European Bioinformatics Institute, Wellcome Trust Genome Campus, Hinxton CB10 1SD, United Kingdom. Electronic address:

Published: November 2013

AI Article Synopsis

  • * These tools comprise a volume viewer for 3D maps, a slice viewer for 2D tomographic slices, and visual analysis pages to help assess the quality of the structures.
  • * The aim is to make it easier for both experts and non-experts to explore and evaluate 3DEM content without needing specialized software or large downloads, while also addressing technical challenges in web accessibility.

Article Abstract

The Protein Data Bank in Europe (PDBe) has developed web-based tools for the visualisation and analysis of 3D electron microscopy (3DEM) structures in the Electron Microscopy Data Bank (EMDB) and Protein Data Bank (PDB). The tools include: (1) a volume viewer for 3D visualisation of maps, tomograms and models, (2) a slice viewer for inspecting 2D slices of tomographic reconstructions, and (3) visual analysis pages to facilitate analysis and validation of maps, tomograms and models. These tools were designed to help non-experts and experts alike to get some insight into the content and assess the quality of 3DEM structures in EMDB and PDB without the need to install specialised software or to download large amounts of data from these archives. The technical challenges encountered in developing these tools, as well as the more general considerations when making archived data available to the user community through a web interface, are discussed.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3898923PMC
http://dx.doi.org/10.1016/j.jsb.2013.09.021DOI Listing

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