Objective: To study the infection status of Leptospira in rodents on Heixiazi island Heilongjiang province in 2011.
Methods: A total of 356 rodents were captured by night trap on the Heixiazi island from April to October 2011. The kidney tissue samples were collected by asepsis operation and the genomic DNA were extracted from them. Leptospira strains were confirmed by polymerase chain reaction(PCR) amplification of the 482 bp 23 S rDNA gene. Fifteen PCR products selected by the month were purified and sequenced by the methods of Sanger dideoxy, the sequences then compared with other Leptospira strains in Genebank, and phylogenetic analyses were drafted by software Mega 4.0.
Results: Among 356 rodents, the dominant species were Clethrionomys rutilus (39.3%, 140/356) and Apodemus agrarius (36.0%, 128/356). The infection rate of Leptospira was 11.0%, with 39 rodent samples detected positive. All the rodent species were infected except for Rattus norvegicus. The infection rate was 9.4% (12/128) in Apodemus agrarius, 12.9%(18/140) in Clethrionomys rutilus, 10.8%(7/65) in Microtus fortis Buchner. No significant difference was found between the infection rate and the species of rodents by chi square test(χ(2) = 1.92, P > 0.05). Among months, the infection rate was 5.6% (4/72) in May, 8.8% (5/57) in June, 12.8% (5/39) in July, 9.8% (5/51) in August, 33.3% (11/33) in September, 22.5% (9/40) in October,but no infection in April. There was significant difference in infection in different months (χ(2) = 32.92, P < 0.05). All the Leptospira in rodents on the Heixiazi island were in the same phylogenetic branch with a high similarity of 97.1%-99.6%, close with the Australia strain U90865 by the similarity above 96.3%.
Conclusion: Leptospira is probably prevalent in rodents on the Heixiazi island, and the phylogene of the strains were similar. The infection rate in rodents was significantly different in months but not in hosts.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Holocene vegetation-hydrology-climate interactions of wetlands on the Heixiazi Island, China.
Sci Total Environ
November 2020
Key Laboratory of Wetland Ecology and Environment, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102, China; Jillin Provincial Joint Laboratory of Changbai Mountain Wetland and Ecology, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102, China.
An integrated view of wetland's evolution is currently poorly understood due to a lack of knowledge on long-term interactions of multiple ecological factors. Here, we present a cored palynological record covering the Holocene Epoch from a depressional wetland on Heixiazi Island (China). With the aid of principal component analysis and cluster analysis of the palynological data, the historical vegetation regime has been well reconstructed for wetlands on the island.
View Article and Find Full Text PDFHantavirus RNA Prevalence in Myomorph Rodents on Bolshoy Ussuriysky Island at the Sino-Russian Border.
Vector Borne Zoonotic Dis
August 2017
1 Public Health College, Harbin Medical University, Harbin, People's Republic of China .
Objective: To understand the distribution and infection status of hantavirus in Myomorph rodents on Bolshoy Ussuriysky Island (Heixiazi Island) at the Sino-Russian border, and to provide data for the safe development and utilization of Bolshoy Ussuriysky Island.
Methods: In 2013 and 2014, Myomorph rodents were trapped on Bolshoy Ussuriysky Island. Total RNA was extracted from rodent tissue, and it was screened for hantavirus RNA by using reverse transcription-polymerase chain reaction.
High prevalence and genetic heterogeneity of rodent-borne Bartonella species on Heixiazi Island, China.
Appl Environ Microbiol
December 2015
State Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping, Beijing, People's Republic of China
We performed genetic analysis of Bartonella isolates from rodent populations from Heixiazi Island in northeast China. Animals were captured at four sites representing grassland and brushwood habitats in 2011 and examined for the prevalence and genetic diversity of Bartonella species, their relationship to their hosts, and geographic distribution. A high prevalence (57.
View Article and Find Full Text PDF[The infection status of Leptospira in rodents on the Heixiazi island of Heilongjiang province, China,in 2011].
Zhonghua Yu Fang Yi Xue Za Zhi
June 2013
Academy of Biological Sciences and Biotechnology, Shenyang Agriculture University,Shenyang 110161,China.
Objective: To study the infection status of Leptospira in rodents on Heixiazi island Heilongjiang province in 2011.
Methods: A total of 356 rodents were captured by night trap on the Heixiazi island from April to October 2011. The kidney tissue samples were collected by asepsis operation and the genomic DNA were extracted from them.
Coxiella burnetii in rodents on Heixiazi Island at the Sino-Russian border.
Am J Trop Med Hyg
April 2013
Institute of Health Quarantine, Chinese Academy of Inspection and Quarantine, Beijing, People's Republic of China.
This work is a molecular epidemiologic study to detect the incidence of Coxiella burnetii in rodents on Heixiazi Island at the Sino-Russian border of Heilongjiang Province. Liver tissues were extracted and processed to test the incidence of C. burnetii infection using polymerase chain reaction analysis.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!