AI Article Synopsis
- Anti-CD3 (aCD3) nanoarrays are a new platform that enhances T cell activation by controlling how the ligand is oriented and its density on the surface.
- Experiments showed that higher aCD3 density on these nanoarrays led to greater CD4+ T cell activation, evidenced by increased CD69 expression, IL-2 production, and cell proliferation.
- Using nanopatterning for aCD3 immobilization resulted in superior T cell activation compared to traditional plastics, allowing more precise adjustments to the T cell response.
Article Abstract
Anti-CD3 (aCD3) nanoarrays fabricated by self-assembled nanopatterning combined with site-directed protein immobilization techniques represent a novel T cell stimulatory platform that allows tight control over ligand orientation and surface density. Here, we show that activation of primary human CD4+ T cells, defined by CD69 upregulation, IL-2 production and cell proliferation, correlates with aCD3 density on nanoarrays. Immobilization of aCD3 through nanopatterning had two effects: cell activation was significantly higher on these surfaces than on aCD3-coated plastics and allowed unprecedented fine-tuning of T cell response.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3834297 | PMC |
| http://dx.doi.org/10.1021/nl4022623 | DOI Listing |
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