AI Article Synopsis
- The study investigates how aspirin causes damage to intestinal epithelial cells and the role of the Multi Drug Resistance (MDR) 1 transporter in this process.
- Researchers used Caco2 cells to assess cell injury after treatment with aspirin, along with verapamil to inhibit MDR1 and observe effects.
- Findings indicate that MDR1 helps in reducing aspirin-induced cell injury, suggesting that the MDR1 transporter plays a significant role in the damage caused by aspirin.
Article Abstract
Background/aims: Although the cytotoxicity of aspirin against the intestinal epithelium is a major clinical problem, little is known about its pathogenesis. We assessed the involvement of Multi Drug Resistance (MDR) 1 in intestinal epithelial cell injury caused by aspirin using MDR1 gene-transfected Caco2 cells.
Methods: Caco2 cells were treated with various concentrations of aspirin for 24 h. After treatment of Caco2 cells with verapamil, a specific inhibitor of MDR1, we assessed the extent of cell injury using a WST-8 assay at 24 h after aspirin-stimulation. We performed the same procedure in MDR1 gene-transfected Caco2 cells. To determine the function of MDR1 in the metabolism of aspirin, flux study was performed using (14)C-labeled aspirin.
Results: The level of aspirin-induced cell injury was higher in verapamil-treated Caco2 cells than in control cells and was less serious in MDR1-transfected Caco2 cells than in control vector-transfected cells. The efflux of (14)C-labeled aspirin was higher in verapamil-treated Caco2 cells than in control cells.
Conclusion: These data suggest that aspirin effux occurs through the MDR1 transporter and that the MDR1 transporter is involved in the pathogenesis of aspirin-induced cell injury.
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| http://dx.doi.org/10.1159/000354497 | DOI Listing |
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