AI Article Synopsis
- IQGAP1 is identified as a new protein that interacts with GDP-bound Rab27a, but this interaction occurs only when Rab27a partners with GTP-bound Cdc42.
- The study shows that IQGAP1 plays a crucial role in regulating the endocytosis of insulin secretory membranes, which is essential for cellular communication.
- When IQGAP1 is silenced, it leads to a decrease in endocytosis and affects the movement of endocytic machinery, suggesting that the activation of Cdc42 by glucose is key for this process at the cell membrane.
Article Abstract
Recruitment of specific molecules to a specific membrane site is essential for communication between specialized membranous organelles. In the present study, we identified IQGAP1 as a novel GDP-bound-Rab27a-interacting protein. We found that IQGAP1 interacts with GDP-bound Rab27a when it forms a complex with GTP-bound Cdc42. We also found that IQGAP1 regulates the endocytosis of insulin secretory membranes. Silencing of IQGAP1 inhibits both endocytosis and the glucose-induced redistribution of endocytic machinery, including Rab27a and its binding protein coronin 3. These processes can also be inhibited by disruption of the trimeric complex with dominant negative IQGAP1 and Cdc42. These results indicate that activation of Cdc42 in response to the insulin secretagogue glucose recruits endocytic machinery to IQGAP1 at the cell periphery and regulates endocytosis at this membrane site.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3889562 | PMC |
| http://dx.doi.org/10.1128/MCB.00895-13 | DOI Listing |
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