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Point-of-care tests for urinary tract infections to reduce antimicrobial resistance: a systematic review and conceptual economic model.
Health Technol Assess
November 2024
Bristol TAG, Population Health Sciences, Bristol Medical School, Bristol, UK.
Background: Urinary tract infections are diagnosed by general practitioners based on symptoms, dipstick tests in some and laboratory urine culture. Patients may be given inappropriate antibiotics. Point-of-care tests can diagnose urinary tract infection in near-patient settings quicker than standard culture.
View Article and Find Full Text PDFPhotonic and electrochemical biosensors for near-patient tests-a critical comparison.
Optica
October 2024
School of Physics, Engineering and Technology and York Biomedical Research Institute, University of York, York, UK.
Research into diagnostic biosensors is a vibrant field that combines scientific challenge with translational opportunities; innovation in healthcare is of great societal interest and is an essential element of future healthcare provision. Photonic and electrochemical biosensors are the dominant modalities, both scientifically and commercially, yet the two scientific communities largely remain separated and siloed. It seems astute to better understand what the two fields can learn from one another so as to progress the key scientific, translational, and commercial challenges.
View Article and Find Full Text PDFA novel machine-learning aided platform for rapid detection of urine ESBLs and carbapenemases: URECA-LAMP.
J Clin Microbiol
November 2024
Department of Pathology & Laboratory Medicine, Medicine, and Microbiology, Immunology and Infectious Diseases, University of Calgary, Calgary, Alberta, Canada.
Pathogenic gram-negative bacteria frequently carry genes encoding extended-spectrum beta-lactamases (ESBL) and/or carbapenemases. Of great concern are carbapenem resistant , , and . Despite the need for rapid AMR diagnostics globally, current molecular detection methods often require expensive equipment and trained personnel.
View Article and Find Full Text PDFA loop-mediated isothermal amplification test for yaws: a multi-country diagnostic accuracy evaluation.
Lancet Glob Health
November 2024
Faculty of Infectious Diseases, London School of Hygiene & Tropical Medicine, London, UK; Hospital for Tropical Diseases, London, UK; Division of Infection and Immunity, University College London, London, UK.
Background: To meet the WHO target of eradicating yaws by 2030, highly sensitive and specific diagnostic tools are needed. A multiplex Treponema pallidum-Haemophilus ducreyi loop-mediated isothermal amplification (TPHD-LAMP) test holds promise as a near-patient diagnostic tool for yaws and H ducreyi. We conducted a prospective evaluation in Cameroon, Côte d'Ivoire, Ghana, and the Republic of the Congo to determine the diagnostic accuracy of the TPHD-LAMP test, as well as to assess its acceptability, feasibility, and cost.
View Article and Find Full Text PDFEvaluation of a near-patient SARS-CoV-2 novel rapid diagnostic platform.
Microbiol Spectr
October 2024
Department of Infection, Immunity and Inflammation, Institute of Child Health, University College London, London, United Kingdom.
The goal of this study is to test a novel device and methodology based on the "Pebble" platform and real-time quantitative colorimetric loop-mediated isothermal amplification (qcLAMP) during SARS-CoV-2 detection using crude samples and extracted RNA. The new method employs an inexpensive lightweight device aimed toward rapid point-of-care testing. An extensive evaluation was performed consisting of 1,693 clinical samples across five independent clinical testing centers.
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