The aim of this study was to investigate the effect of XAD4-column treatment on removal of several fermentation inhibitors from corncob hydrolysate (CH). From analysis using a model hydrolysate, more than 99% of 5-hydroxy-methyl furfural, furfural and vanillin were removed by this treatment, and more than 97% of the total xylose, glucose and arabinose remained in the detoxified CH (DCH). The resulting DCH was tested as a substrate for ethanol production by Saccharomyces cerevisiae and Pichia stipitis. The highest ethanol levels for S. cerevisiae were 1.40 and 4.92 g l(-1) in CH and DCH, respectively. For P. stipitis, the levels were 0 and 4.73 g l(-1) in the CH and DCH media, respectively. The levels of alcohol volumetric productivity in the DCH medium were 0.374 and 0.200 g l(-1)h(-1) for S. cerevisiae and P. stipitis, respectively.
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http://dx.doi.org/10.1016/j.biortech.2013.08.166 | DOI Listing |
Nat Commun
November 2024
State Key Laboratory of Biocatalysis and Enzyme Engineering, Environmental Microbial Technology Center of Hubei Province, and School of Life Sciences, Hubei University, Wuhan, China.
Metab Eng
January 2025
Key Laboratory of Quantitative Synthetic Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China. Electronic address:
Inducible transcription systems are essential tools in genetic engineering, where tight control, strong inducibility and fast response with cost-effective inducers are highly desired. However, existing systems in yeasts are rarely used in large-scale fermentations due to either cost-prohibitive inducers or incompatible performance. Here, we developed powerful xylose and arabinose induction systems in Saccharomyces cerevisiae, utilizing eukaryotic activators XlnR and AraR from Aspergillus species and bacterial repressors XylR and AraR.
View Article and Find Full Text PDFFungal Biol Biotechnol
October 2024
Metabolic Engineering Group, Department of Microbiology and Genetics, Universidad de Salamanca, Campus Miguel de Unamuno, 37007, Salamanca, Spain.
Background: Ashbya gossypii is a filamentous fungus widely utilized for industrial riboflavin production and has a great potential as a microbial chassis for synthesizing other valuable metabolites such as folates, biolipids, and limonene. Engineered strains of A. gossypii can effectively use various waste streams, including xylose-rich feedstocks.
View Article and Find Full Text PDFSci Rep
October 2024
Center of Excellence in Agro Bio-Circular-Green Industry (Agro BCG), Faculty of Agro-Industry, Chiang Mai University, Chiang Mai, 50100, Thailand.
A co-substrate model of Candida tropicalis TISTR 5306 cultivated in 10 - 100 g/L xylose and 1 - 10 g/L glucose at the ratio of 10:1 was developed based in part on modified Monod equation. The kinetic parameters include substrate limitation as well as substrate and product inhibitions with inclusion of threshold values. A general good fitting with average RSS, R, and MS values of 162, 0.
View Article and Find Full Text PDFMicrob Cell Fact
October 2024
Anhui Province Key Laboratory of Pollutant Sensitive Materials and Environmental Remediation, School of Life Sciences, Huaibei Normal University, Huaibei, 235000, Anhui, P. R. China.
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