AI Article Synopsis
- Learned associations are encoded within neuronal ensembles, but proving they mediate learned behaviors has been challenging due to the limitations of traditional methods that impact all neurons.
- New approaches, including Daun02 inactivation, FACS sorting, and Fos-GFP transgenic rats, allow for selective targeting and analysis of activated neurons in the context of drug effects and relapse.
- Additional tools like Fos-tTA transgenic mice and CREB-overexpressing neuron inactivation help investigate the role of these neuronal ensembles in conditioned fear.
Article Abstract
Correlational data suggest that learned associations are encoded within neuronal ensembles. However, it has been difficult to prove that neuronal ensembles mediate learned behaviours because traditional pharmacological and lesion methods, and even newer cell type-specific methods, affect both activated and non-activated neurons. In addition, previous studies on synaptic and molecular alterations induced by learning did not distinguish between behaviourally activated and non-activated neurons. Here, we describe three new approaches--Daun02 inactivation, FACS sorting of activated neurons and Fos-GFP transgenic rats--that have been used to selectively target and study activated neuronal ensembles in models of conditioned drug effects and relapse. We also describe two new tools--Fos-tTA transgenic mice and inactivation of CREB-overexpressing neurons--that have been used to study the role of neuronal ensembles in conditioned fear.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4530016 | PMC |
| http://dx.doi.org/10.1038/nrn3597 | DOI Listing |
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