Clotting factor VIII (FVIII) and thrombin generation in camel plasma: A comparative study with humans.

Can J Vet Res

The Coagulation Research Laboratory, Physiology Department, College of Medicine and King Khalid University Hospital (Gader, Al Momen, Alhaider) and College of Agriculture and Food Sciences (Al Haidary, Hussain), King Saud University, Riyadh 11461, Saudi Arabia; Comparative Coagulation Section, Animal Health Diagnostic Center, Cornell University, Ithaca, New York, USA (Brooks, Catalfamo).

Published: April 2013

The objective of this study was to characterize the highly elevated levels of clotting factor VIII (FVIII) in camel plasma. Whole blood was collected from healthy camels and factor VIII clotting activity (FVIII:C) assays were conducted using both the clotting and the chromogenic techniques. The anticoagulant citrate phosphate dextrose adenine (CPDA) produced the highest harvest of FVIII:C, the level of plasma factor VIII, compared to heparin:saline and heparin:CPDA anticoagulants. Camel FVIII can be concentrated 2 to 3 times in cryoprecipitate. There was a significant loss of camel FVIII when comparing levels of FVIII in camel plasma after 1 h of incubation at 37°C (533%), 40°C (364%), and 50°C (223%). Thrombin generation of camel plasma is comparable to that of human plasma. It was concluded that camel plasma contains very elevated levels of FVIII:C, approaching 8 times the levels in human plasma, and that these elevated levels could not be attributed to excessive thrombin generation. Unlike human FVIII:C, camel FVIII:C is remarkably heat stable. Taken together, these unique features of camel FVIII could be part of the physiological adaptation of hemostasis of the Arabian camel in order to survive in the hot desert environment.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3605932PMC

Publication Analysis

Top Keywords

camel plasma
20
factor viii
16
thrombin generation
12
elevated levels
12
camel fviii
12
camel
10
clotting factor
8
viii fviii
8
generation camel
8
plasma
8

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!