AI Article Synopsis
- The genomic sequence of the Helicoverpa armigera nucleopolyhedrovirus from Australia (HearNPV-Au) was analyzed, revealing a genome size of 130,992 bp and 134 predicted open reading frames (ORFs).
- HearNPV-Au shares a high level of genetic similarity with both AcMNPV and HearSNPV-G4, with 94 common ORFs, and exhibits a nucleotide sequence identity of 99% with HearSNPV-G4.
- The study identified major genetic differences between HearNPV-Au and related viruses in homologous regions and baculovirus repeat ORF genes, confirming that these two viruses belong to the same species despite some unique gene variations
Article Abstract
The complete genomic sequence of Helicoverpa armigera nucleopolyhedrovirus from Australia, HearNPV-Au, was determined and analyzed. The HearNPV-Au genome was 130,992 bp in size with a G+C content of 39 mol% and contained 134 predicted open reading frames (ORFs) consisting of more than 150 nucleotides. HearNPV-Au shared 94 ORFs with AcMNPV, HearSNPV-G4 and SeMNPV, and was most closely related to HearSNPV-G4. The nucleotide sequence identity between HearNPV-Au and HearSNPV-G4 genome was 99%. The major differences were found in homologous regions (hrs) and baculovirus repeat ORFs (bro) genes. Five hrs and two bro genes were identified in the HearNPV-Au genome. All of the 134 ORFs identified in HearNPV-Au were also found in HearSNPV-G4, except the homologue of ORF59 (bro) in HearSNPV-G4. The sequence data strongly suggested that HearNPV-Au and HearSNPV-G4 belong to the same virus species.
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| http://dx.doi.org/10.1007/s00705-013-1823-3 | DOI Listing |
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