The prerequisite to monitor gene expression is the selection of reference genes for normalization of RT-qPCR results. Using 13 sputum samples collected from 9 CF patients, we demonstrated that PA2875 and PA3340 are better reference genes than the previously used clpX and oprL genes.
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Background: The photothermal sensitivity of tobacco refers to how tobacco plants respond to variations in the photothermal conditions of their growth environment. The degree of this sensitivity is crucial for determining the optimal planting regions for specific varieties, as well as for improving the quality and yield of tobacco leaves. However, the precise mechanisms underlying the development of photothermal sensitivity in tobacco remain unclear.
View Article and Find Full Text PDFBrCYP71 mutation resulted in stay-green in pak choi (Brassica rapa L. ssp. chinensis).
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College of Horticulture, Shenyang Agricultural University, Shenyang, 110866, People's Republic of China.
BrCYP71 encoding multifunctional oxidase was mapped using BSA-Seq and linkage analysis, and its function in stay-green of pak choi was verified through Arabidopsis heterologous transgenic experiment. Stay-green refers to the phenomenon that plant leaves remain green during senescence and even after death, which is of great significance for improving the commerciality of leafy vegetables during storage or transportation and extending their shelf life. In this study, we identified a stay-green mutant of pak choi and named it nye2.
View Article and Find Full Text PDFPopulation-based, first-tier genomic newborn screening in the maternity ward.
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Division of Child Neurology, Reference Center for Neuromuscular Diseases, Department of Pediatrics, CHU Liege, University of Liege, Liege, Belgium.
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View Article and Find Full Text PDFAuthor Correction: Selection of suitable reference genes for gene expression studies in HMC3 cell line by quantitative real-time RT-PCR.
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Department for Life Quality Studies - QUVI, University of Bologna, Rimini, Italy.
Investigating the role and mechanisms of bisphenol compounds in premature ovarian insufficiency using computational biology and bioinformatics.
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Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, China; National Clinical Research Center for Obstetrics and Gynecology (Peking University Third Hospital), Beijing, China. Electronic address:
Premature Ovarian Insufficiency refers to the premature decline in ovarian function before the age of 40, resulting in menstrual irregularities or complete cessation of menstruation, and affecting fertility. Widely used bisphenol compounds may have potential health effects, including premature ovarian insufficiency (POI). This study employs computational biology and bioinformatics to investigate the effects of bisphenols (BPs) on POI.
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