We present a seminested PCR method that specifically discriminates between Plasmodium ovale curtisi and P. ovale wallikeri with high sensitivity. The test is based on species-specific amplification of a size-polymorphic fragment of the tryptophan-rich antigen gene, potra, which also permits discrimination of intraspecific sequence variants at this locus.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3838052PMC
http://dx.doi.org/10.1128/JCM.01527-13DOI Listing

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