The distribution of norovirus (NoV) genogroup II in children < 5 years of age admitted to a south Indian hospital with diarrhea was investigated. Viral RNA extracted from 282 stool samples were screened for NoV GII and positive amplicons sequenced. Twenty-eight (9.9%) had NoV GII infection with a median age of 6 months, with more severe episodes of diarrhea among infected (median Vesikari score 13, interquartile range [IQR] 10-15) than children without infection (median score 10, IQR 8-13, P = 0.002). The study documents NoV GII infections as an important cause of gastroenteritis and the genetic diversity of circulating strains.
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http://dx.doi.org/10.4269/ajtmh.13-0121 | DOI Listing |
Viruses
November 2024
Division of Water Supply and Sewerage Research, National Institute of Environmental Research, Incheon 22689, Republic of Korea.
Groundwater is an essential drinking water source for humans. However, improper groundwater management leads to fecal contamination and waterborne diseases caused by viral pathogens. Therefore, this study aimed to investigate norovirus (NoV) contamination by conducting nationwide monitoring over five years (2019-2023).
View Article and Find Full Text PDFArch Virol
January 2025
Center for Translational Medicine, Affiliated Infectious Diseases Hospital of Zhengzhou University (Henan Infectious Diseases Hospital, The Sixth People's Hospital of Zhengzhou), Zhengzhou, 450000, People's Republic of China.
Trypsin digestion of the GII.6 norovirus (NoV) major capsid protein VP1 promotes its binding to histo-blood group antigens (HBGAs), which are believed to be co-receptors for NoVs. In our previous study, we found that trypsin digestion led to the disassembly of GII.
View Article and Find Full Text PDFAnal Methods
January 2025
The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, People's Republic of China.
Appl Environ Microbiol
December 2024
School of Public Health, Hebei Medical University, Shijiazhuang, China.
To achieve rapid and simultaneous detection of NoV GI, NoV GII, and HAV, a quadruple real-time fluorescence quantitative PCR (RT-qPCR) assay was developed using MS2 bacteriophage as a process control virus. The quadruple RT-qPCR assay effectively detected NoV GI, NoV GII, HAV, and MS2 RNA with detection limits of 10 copies/μL, 10 copies/μL, 10 copies/μL, and 10 copies/μL, respectively, within 1 hour 50 minutes. The quadruple RT-qPCR assay could specifically detect NoV GI, NoV GII, HAV, and MS2 without cross-reactions with other common pathogens, demonstrating good reproducibility with intra-assay and inter-assay coefficients of variation all below 2.
View Article and Find Full Text PDFMolecules
November 2024
CERES, Microbiology Laboratory, Faculty of Pharmacy, University of Coimbra, Pólo das Ciências da Saúde, 3000-548 Coimbra, Portugal.
The increased presence of resistant microorganisms in water promotes the need for supplementary measures to mitigate the water source's contamination. Traditional treatments are inefficient in wastewater management at removing some emerging contaminants. an invasive species, can be used in the treatment due to their resistance and biofiltration capacity, working as a pest management strategy.
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